Characterization of the ros1-gene products expressed in human glioblastoma cell lines

Sharma, S., Birchmeier, C., Nikawa, J., O'Neill, K., Rodgers, L., Wigler, M. H. (1989) Characterization of the ros1-gene products expressed in human glioblastoma cell lines. Oncogene Res, 5 (2). pp. 91-100. ISSN 0890-6467

URL: https://www.ncbi.nlm.nih.gov/pubmed/2691958

Abstract

Most glioblastoma-derived cell lines express an 8.3 kb ros1 transcript and a 280 kD glycoprotein designated gp280ros1, which can be specifically immunoprecipitated with an anti-ROS antibody. This 280 kD protein possesses in vitro autokinase activity and was observed in four independent glioblastoma cell lines. In a fifth glioblastoma cell line, U-118 MG, a smaller ros1 transcript of 4.0 kb was observed. Immunoprecipitation analysis reveals that the U-118 MG expressed a smaller, 116 kD ros1 gene product. cDNA cloning and sequencing of the U-118 MG ros1 transcript indicates it encodes the entire tyrosine kinase domain and two amino acids of the transmembrane domain of ros1 at its 3' end. Sequences at its 5' end likely arise from another gene.

Item Type: Paper
Additional Information:
Uncontrolled Keywords: Amino Acid Sequence Base Sequence DNA analysis Glioma analysis Humans Molecular Sequence Data Precipitin Tests Proto-Oncogene Proteins analysis Proto-Oncogenes Transcription Genetic Tumor Cells Cultured
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > oncogene
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase > tyrosine kinase
CSHL Authors:
Communities: CSHL labs > Wigler lab
Depositing User: CSHL Librarian
Date: 1989
Date Deposited: 05 Apr 2012 18:56
Last Modified: 08 Nov 2016 21:16
URI: http://repository.cshl.edu/id/eprint/26011

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