Design of a retroviral-mediated ecdysone-inducible system and its application to the expression profiling of the PTEN tumor suppressor

Stolarov, J., Chang, K., Reiner, A., Rodgers, L., Hannon, G. J., Wigler, M. H., Mittal, V. (November 2001) Design of a retroviral-mediated ecdysone-inducible system and its application to the expression profiling of the PTEN tumor suppressor. Proceedings of the National Academy of Sciences of the United States of America, 98 (23). pp. 13043-13048. ISSN 0027-8424

URL: https://www.ncbi.nlm.nih.gov/pubmed/11687610
DOI: 10.1073/pnas.221450598

Abstract

We have engineered the ecdysone-inducible mammalian expression system for general retroviral delivery to cultured mammalian cells. We inducibly expressed PTEN in the glioblastoma cell line, U87MG, lacking this gene. Because nearly all cells are recruited on induction, we find both up- and down-regulated genes by cDNA microarray analysis. The changes we see are similar to those observed after treatment with LY294002, an inhibitor of phosphatidylinositol 3-OH kinase, fully consistent with the model that PTEN antagonizes phosphatidylinositol 3-OH kinase. Both treatments result in suppressed expression of the transforming growth factor (TGF)-beta gene and the genes of the cholesterol biosynthesis pathway. Our results illustrate the power of using a fully inducible expression system in conjunction with cDNA microarray analysis for exploring gene function.

Item Type: Paper
Uncontrolled Keywords: inducible expression glioblastoma DNA microarray GENE-EXPRESSION gene expression MAMMALIAN-CELLS mammalian cells COWDEN-DISEASE Cowden disease GERMLINE MUTATIONS germline mutations TRANSGENIC MICE transgenic mice PHOSPHATASE phosphate VECTORS vectors BREAST breast cancer CANCER PTEN/MMAC1 PTEN MMAC1
Subjects: bioinformatics > genomics and proteomics > analysis and processing > microarray gene expression processing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > PTEN
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > cDNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene transfer
organism description > virus
CSHL Authors:
Communities: CSHL labs > Hannon lab
CSHL labs > Mittal lab
CSHL labs > Wigler lab
Depositing User: CSHL Librarian
Date: November 2001
Date Deposited: 05 Apr 2012 18:31
Last Modified: 02 Nov 2016 16:20
PMCID: PMC60821
Related URLs:
URI: http://repository.cshl.edu/id/eprint/26005

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