Complex formation between RAS and RAF and other protein kinases

Van Aelst, L., Barr, M., Marcus, S., Polverino, A., Wigler, M. H. (July 1993) Complex formation between RAS and RAF and other protein kinases. Proc Natl Acad Sci U S A, 90 (13). pp. 6213-7. ISSN 0027-8424 (Print)

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URL: https://www.ncbi.nlm.nih.gov/pubmed/8327501
DOI: 10.1073/pnas.90.13.6213

Abstract

We used a Saccharomyces cerevisiae genetic system to detect the physical interaction of RAS and RAF oncoproteins. We also observed interaction between RAS and byr2, a protein kinase implicated as a mediator of the Schizosaccharomyces pombe ras1 protein. Interaction with RAS required only the N-terminal domains of RAF or byr2 and was disrupted by mutations in either the guanine nucleotide-binding or effector-loop domains of RAS. We observed interaction between MEK (a kinase that phosphorylates mitogen-activated protein kinases) and the catalytic domain of RAF. RAS and MEK also interacted but only when RAF was overexpressed.

Item Type: Paper
Uncontrolled Keywords: Base Sequence Fungal Proteins metabolism GTP-Binding Proteins metabolism Guanosine Triphosphate metabolism MAP Kinase Kinase 1 Mitogen-Activated Protein Kinase Kinases Molecular Sequence Data Protein Kinases metabolism Protein-Serine Threonine Kinases metabolism Protein Tyrosine Kinase metabolism Proto-Oncogene Proteins metabolism Proto-Oncogene Proteins c-raf Proto-Oncogene Proteins p21(ras) metabolism Saccharomyces cerevisiae metabolism Saccharomyces cerevisiae Proteins Schizosaccharomyces metabolism Schizosaccharomyces pombe Proteins ras Proteins
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > RAS
organism description > yeast > Saccharomyces
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > oncogene
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase > tyrosine kinase
CSHL Authors:
Communities: CSHL labs > Van Aelst lab
CSHL labs > Wigler lab
Depositing User: CSHL Librarian
Date: 1 July 1993
Date Deposited: 06 Apr 2012 13:56
Last Modified: 09 Nov 2017 19:59
PMCID: PMC46898
Related URLs:
URI: http://repository.cshl.edu/id/eprint/25991

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