byr2, a Schizosaccharomyces pombe gene encoding a protein kinase capable of partial suppression of the ras1 mutant phenotype

Wang, Y., Xu, H. P., Riggs, M., Rodgers, L., Wigler, M. H. (July 1991) byr2, a Schizosaccharomyces pombe gene encoding a protein kinase capable of partial suppression of the ras1 mutant phenotype. Molecular & Cellular Biology, 11 (7). pp. 3554-63. ISSN 0270-7306

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URL: https://www.ncbi.nlm.nih.gov/pubmed/2046669
DOI: 10.1128/MCB.11.7.3554

Abstract

Schizosaccharomyces pombe contains a single gene, ras1, which is a homolog of the mammalian RAS genes. ras1 is required for conjugation, sporulation, and normal cell shape. ras1 has been previously identified as ste5. We report here a gene we call byr2 that can encode a predicted protein kinase and can partially suppress defects in ras1 mutants. ras1 mutant strains expressing high levels of byr2 can sporulate competently but are still defective in conjugation and abnormally round. byr2 mutants are viable and have normal shape but are absolutely defective in conjugation and sporulation. byr2 is probably identical to ste8. In many respects, byr2 resembles the byr1 gene, another suppressor of the ras1 mutation, which has been identified previously as ste1. Our data indicate that if ras1, byr2, and byr1 act along the same pathway, then the site of action for byr2 is between the sites for ras1 and byr1.

Item Type: Paper
Additional Information: Journal Article
Uncontrolled Keywords: Amino Acid Sequence Base Sequence Comparative Study Genes Structural Fungal Genes ras Genotype Molecular Sequence Data Mutagenesis Site-Directed Oligonucleotide Probes Phenotype Polymerase Chain Reaction Protein Kinases genetics Restriction Mapping Schizosaccharomyces cytology enzymology genetics Sequence Homology, Nucleic Acid Suppression Genetic
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > RAS
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene regulation
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene regulation

bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase
CSHL Authors:
Communities: CSHL labs > Wigler lab
Depositing User: CSHL Librarian
Date: July 1991
Date Deposited: 06 Apr 2012 15:14
Last Modified: 08 Nov 2016 21:14
PMCID: PMC361098
Related URLs:
URI: http://repository.cshl.edu/id/eprint/25985

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