Multiple Ras functions can contribute to mammalian cell transformation

White, M. A., Nicolette, C., Minden, A., Polverino, A., Van Aelst, L., Karin, M., Wigler, M. H. (February 1995) Multiple Ras functions can contribute to mammalian cell transformation. Cell, 80 (4). pp. 533-41. ISSN 0092-8674 (Print)

URL: https://www.ncbi.nlm.nih.gov/pubmed/7867061
DOI: 10.1016/0092-8674(95)90507-3

Abstract

We have developed a generalized approach, using two hybrid interactions, to isolate Ha-Ras effector loop mutations that separate the ability of Ha-Ras to interact with different downstream effectors. These mutations attenuate or eliminate Ha-ras(G12V) transformation of mammalian cells, but retain complementary activity, as demonstrated by synergistic induction of foci of growth-transformed cells, and by the ability to activate different downstream components. The transformation defect of Ha-ras(G12V, E37G) is rescued by a mutant, raf1, that restores interaction. These results indicate that multiple cellular components, including Raf1, are activated by Ha-Ras and contribute to Ha-Ras-induced mammalian cell transformation.

Item Type: Paper
Uncontrolled Keywords: Animals Cell Transformation Neoplastic genetics Cells Cultured Chloramphenicol O-Acetyltransferase analysis Gene Library Genes, ras genetics Genetic Complementation Test Humans Mice Mutation Protein Kinases analysis Protein Serine Threonine Kinases genetics Proto-Oncogene Proteins genetics Proto-Oncogene Proteins c-raf Rats Recombinant Fusion Proteins biosynthesis Saccharomyces cerevisiae genetics Schizosaccharomyces genetics Transfection
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > RAS
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > oncogene
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein expression
CSHL Authors:
Communities: CSHL labs > Van Aelst lab
CSHL labs > Wigler lab
CSHL labs > Stillman lab
Depositing User: CSHL Librarian
Date: 24 February 1995
Date Deposited: 06 Apr 2012 14:55
Last Modified: 18 Nov 2016 17:08
Related URLs:
URI: http://repository.cshl.edu/id/eprint/25981

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