The somatic replication of DNA methylation

Wigler, M. H., Levy, D., Perucho, M. (1981) The somatic replication of DNA methylation. Cell, 24 (1). pp. 33-40.

URL: https://www.ncbi.nlm.nih.gov/pubmed/6263490
DOI: 10.1016/0092-8674(81)90498-0

Abstract

The hypothesis that DNA methylation patterns are replicated in the somatic cells of vertebrates was tested. M-Hpa II, the modification enzyme from Hemophilus parainfluenzae, was used to methylate bacteriophage .vphi.X174 RF DNA and the cloned chicken thymidine kinase (tk) gene in vitro and then to introduce these DNA and unmethylated controls into tk- cultured mouse cells by DNA-mediated transformation. Twenty-five cell generations later, the state of methylation of transferred DNA was examined by restriction endonuclease analysis and blot hybridization. Methylation at Hpa II sites is replicated by these cultured cells but not with 100% fidelity. Methylation of the cloned chicken tk gene decreases its apparent transformation efficiency relative to unmethylated molecules.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA methylation
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication
CSHL Authors:
Communities: CSHL labs > Wigler lab
Depositing User: CSHL Librarian
Date Deposited: 06 Apr 2012 15:56
Last Modified: 18 Nov 2016 17:00
Related URLs:
URI: http://repository.cshl.edu/id/eprint/25966

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