DNA-Mediated Transfer of the Adenine Phosphoribosyltransferase Locus into Mammalian Cells

Wigler, M. H., Pellicer, A., Silverstein, S., Axel, R., Urlaub, G., Chasin, L. (March 1979) DNA-Mediated Transfer of the Adenine Phosphoribosyltransferase Locus into Mammalian Cells. Proc Natl Acad Sci U S A, 76 (3). pp. 1373-1376.

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URL: http://www.ncbi.nlm.nih.gov/pubmed/286319
DOI: 10.1073/pnas.76.3.1373

Abstract

In this report, we demonstrate the feasibility of transforming mouse cells deficient in adenine phosphoribosyltransferase (aprt; AMP:pyrophosphate phosphoribosyltransferase, EC 2.4.2.7) to the aprt+ phenotype by means of DNA-mediated gene transfer. Transformation was effected by using unfractionated high molecular weight genomic DNA from Chinese hamster, human, and mouse cells and restriction endonuclease-digested DNA from rabbit liver. The transformation frequency observed was between 1 and 10 colonies per 106 cells per 20 {micro} g of donor DNA. Transformants displayed enzymatic activity that was donor derived as demonstrated by isoelectric focusing of cytoplasmic extracts. These transformants fall into two classes: those that are phenotypically stable when grown in the absence of selective pressure and those that are phenotypically unstable under the same conditions.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene transfer
CSHL Authors:
Communities: CSHL labs > Wigler lab
Depositing User: CSHL Librarian
Date: 1 March 1979
Date Deposited: 06 Apr 2012 15:47
Last Modified: 09 Nov 2017 21:00
PMCID: PMC383253
Related URLs:
URI: http://repository.cshl.edu/id/eprint/25963

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