Biological activity of the mammalian RAP genes in yeast

Xu, H. P., Wang, Y., Riggs, M., Rodgers, L., Wigler, M. H. (September 1990) Biological activity of the mammalian RAP genes in yeast. Cell Regulation, 1 (10). pp. 763-769. ISSN 1044-2030

URL: https://www.ncbi.nlm.nih.gov/pubmed/1983087

Abstract

We have screened expression libraries for mammalian cDNAs capable of suppressing defects in ras1- Schizosaccharomyces pombe. Both the RAP1A and RAP1B genes were identified in this manner. They suppress defects in cell morphology and sporulation, although not conjugation. In contrast, RAP genes do not suppress phenotypes in the yeast Saccharomyces cerevisiae that are deficient in RAS. Indeed, expression of RAP1A appears to antagonize the activated S. cerevisiae RAS2val19 gene. These results indicate that RAP proteins can interact with RAS targets, sometimes productively, sometimes nonproductively.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > RAS
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
CSHL Authors:
Communities: CSHL labs > Stillman lab
CSHL labs > Wigler lab
Depositing User: CSHL Librarian
Date: September 1990
Date Deposited: 06 Apr 2012 18:42
Last Modified: 27 Feb 2017 14:59
PMCID: PMC361659
URI: http://repository.cshl.edu/id/eprint/25948

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