Characterization of the rat mas oncogene and its high-level expression in the hippocampus and cerebral cortex of rat brain

Young, D., O'Neill, K., Jessell, T., Wigler, M. H. (July 1988) Characterization of the rat mas oncogene and its high-level expression in the hippocampus and cerebral cortex of rat brain. Proc Natl Acad Sci U S A, 85 (14). pp. 5339-42. ISSN 0027-8424

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URL: https://www.ncbi.nlm.nih.gov/pubmed/2455902
DOI: 10.1073/pnas.85.14.5339

Abstract

The human mas oncogene was originally detected by its ability to transform NIH 3T3 cells. We previously showed that the protein encoded by this gene is unique among cellular oncogene products in that it has seven hydrophobic potential transmembrane domains and shares strong sequence similarity with a family of hormone-receptor proteins. We have now cloned the rat homolog of the mas oncogene, determined its DNA sequence, and examined its expression in various rat tissues. A comparison of the predicted sequences of the rat and human mas proteins shows that they are highly conserved, except in their hydrophilic amino-terminal domains. Our examination of the expression of mas, determined by RNA-protection studies, indicates that high levels of mas RNA transcripts are present in the hippocampus and cerebral cortex of the brain, but not in other neural regions or in other tissues. This pattern of expression and the similarity of mas protein to known receptor proteins suggest that mas encodes a receptor that is involved in the normal neurophysiology and/or development of specific neural tissues.

Item Type: Paper
Uncontrolled Keywords: Amino Acid Sequence Animals Base Sequence Brain metabolism Cerebral Cortex metabolism Cloning Molecular Comparative Study DNA Recombinant Hippocampus metabolism Humans Membrane Proteins Molecular Sequence Data Neoplasm Proteins genetics Proto-Oncogene Proteins genetics Proto Oncogenes RNA genetics Rats Rats Inbred Strains Tissue Distribution Transcription, Genetic
Subjects: organs, tissues, organelles, cell types and functions > tissues types and functions > cerebral cortex
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
organs, tissues, organelles, cell types and functions > tissues types and functions > hippocampus
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > oncogene
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein characterization
organism description > animal > mammal > rodent > rat
organism description > animal > mammal > rodent > rat
CSHL Authors:
Communities: CSHL labs > Wigler lab
Depositing User: CSHL Librarian
Date: July 1988
Date Deposited: 10 Apr 2012 14:12
Last Modified: 09 Nov 2017 20:55
PMCID: PMC281746
Related URLs:
URI: http://repository.cshl.edu/id/eprint/25944

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