Leaf senescence is delayed in tobacco plants expressing the maize homeobox gene knotted1 under the control of a senescence-activated promoter

Ori, N., Juarez, M. T., Jackson, D. P, Yamaguchi, J., Banowetz, G. M., Hake, S. (1999) Leaf senescence is delayed in tobacco plants expressing the maize homeobox gene knotted1 under the control of a senescence-activated promoter. Plant Cell, 11 (6). pp. 1073-1080. ISSN 10404651 (ISSN)

URL: https://www.ncbi.nlm.nih.gov/pubmed/10368178
DOI: 10.1105/tpc.11.6.1073

Abstract

Leaf senescence is an active process involving remobilization of nutrients from senescing leaves to other parts of the plant. Whereas senescence is accompanied by a decline in leaf cytokinin content, supplemental cytokinin delays senescence. Plants that overexpress isopentenyl transferase (ipt), a cytokinin-producing gene, or knotted1 (kn1), a homeobox gene, have many phenotypes in common. Many of these phenotypes are characteristic of altered cytokinin physiology. The effect of kn1 on leaf senescence was tested by driving its expression using the promoter of the senescence-associated gene SAG12. SAG:kn1 tobacco plants showed a marked delay in leaf senescence but otherwise developed normally. The delay in senescence was revealed by an increase in chlorophyll content in SAG:kn1 leaves relative to leaves of the control plants and by a decrease in the number of dead leaves. Senescence was also delayed in detached leaves of SAG:kn1 plants. Delayed senescence was accompanied by increased leaf cytokinin content in older leaves expressing kn1. These experiments extend the current understanding of kn1 function and suggest that in addition to mediating meristem maintenance, kn1 is capable of regulating the onset of senescence in leaves.

Item Type: Paper
Uncontrolled Keywords: adenylate isopentenyltransferase cytokinin homeodomain protein Kn1 protein plant transferase vegetable protein aging article biosynthesis genetics growth, development and aging maize metabolism physiology plant plant leaf promoter region tobacco transgenic plant Alkyl and Aryl Transferases Cytokinins Homeodomain Proteins Plant Leaves Plant Proteins Plants, Genetically Modified Plants, Toxic Promoter Regions Genetics Zea mays Embryophyta Nicotiana obtusifolia Nicotiana tabacum
Subjects: organism description > plant
organism description > plant behavior > plant orientation
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > senescence
CSHL Authors:
Communities: CSHL labs > Jackson lab
Depositing User: CSHL Librarian
Date: 1999
Date Deposited: 03 Apr 2012 17:44
Last Modified: 12 Jan 2017 16:02
PMCID: PMC144237
Related URLs:
URI: http://repository.cshl.edu/id/eprint/25939

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving