Intercellular trafficking of a KNOTTED1 green fluorescent protein fusion in the leaf and shoot meristem of Arabidopsis

Kim, J. Y., Yuan, Z. A., Cilia, M., Khalfan-Jagani, Z., Jackson, D. P. (March 2002) Intercellular trafficking of a KNOTTED1 green fluorescent protein fusion in the leaf and shoot meristem of Arabidopsis. Proceedings of the National Academy of Sciences of the United States of America, 99 (6). pp. 4103-4108. ISSN 0027-8424

URL: http://www.ncbi.nlm.nih.gov/pubmed/11891300
DOI: 10.1073/pnas.052484099

Abstract

Dominant mutations in the maize homeobox gene knotted1 (kn1) act nonautonomously during maize leaf development, indicating that Kn1 is involved in the generation or transmission of a developmental signal that passes from the inner layers of the leaf to epidermal cells. We previously found that this nonautonomous activity is correlated with the presence of KN1 protein in leaf epidermal cells, where KN1 mRNA could not be detected. Furthermore, KN1 protein expressed in Escherichia coli and labeled with a fluorescent dye can traffic between leaf mesophyll cells in microinjection assays. Here we show that green fluorescent protein (GFP)-tagged KN1 is able to traffic between epidermal cells of Arabidopsis and onion. When expressed in vivo, the GFP approximately KN1 fusion trafficked from internal tissues of the leaf to the epidermis, providing the first direct evidence, to our knowledge, that KN1 can traffic across different tissue layers in the leaf. Control GFP fusions did not show this intercellular trafficking ability. GFP approximately KN1 also trafficked in the shoot apical meristem, suggesting that cell-to-cell trafficking of KN1 may be involved in its normal function in meristem initiation and maintenance.

Item Type: Paper
Subjects: organism description > plant > Arabidopsis
organism description > plant > maize
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > green fluorescent protein
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > knotted1
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > knotted1
CSHL Authors:
Communities: CSHL labs > Jackson lab
Watson School > Publications
Depositing User: CSHL Librarian
Date: March 2002
Date Deposited: 03 Apr 2012 18:00
Last Modified: 19 Sep 2014 14:09
PMCID: PMC122655
Related URLs:
URI: http://repository.cshl.edu/id/eprint/25930

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