Double labeling of KNOTTED1 mRNA and protein reveals multiple potential sites of protein trafficking in the shoot apex

Jackson, D. P. (August 2002) Double labeling of KNOTTED1 mRNA and protein reveals multiple potential sites of protein trafficking in the shoot apex. Plant Physiology, 129 (4). pp. 1423-1429. ISSN 0032-0889

URL: https://www.ncbi.nlm.nih.gov/pubmed/12177455
DOI: 10.1104/pp.006049

Abstract

Recent reports indicate that several plant mRNAs and proteins are able to traffic intercellularly through plasmodesmata. Localization studies can reveal differences between mRNA and protein localization that would be indicative of such a process. However, subtle differences could be missed when comparing localization in adjacent sections, especially in developmental studies where adjacent sections through immature apical regions may be one or more cells removed from each other. Therefore, we have developed a novel method for double localization of KNOTTED1 mRNA and protein in sections through the maize (Zea mays) shoot apex. The advantage of double labeling is revealed in our demonstration of novel potential sites of cell-to-cell trafficking of KNOTTED1 protein in the shoot apical region. The technique should be applicable to any gene products where the appropriate probes are available and will, therefore, help to determine the extent of protein and/or mRNA trafficking in plants.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > knotted1
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > knotted1

bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mRNA dynamics
CSHL Authors:
Communities: CSHL labs > Jackson lab
Depositing User: CSHL Librarian
Date: August 2002
Date Deposited: 03 Apr 2012 20:28
Last Modified: 11 Jan 2017 21:49
PMCID: PMC1540245
Related URLs:
URI: http://repository.cshl.edu/id/eprint/25914

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