Analysis of the competence to respond to KNOTTED1 activity in Arabidopsis leaves using a steroid induction system

Hay, A., Jackson, D. P., Ori, N., Hake, S. (2003) Analysis of the competence to respond to KNOTTED1 activity in Arabidopsis leaves using a steroid induction system. Plant Physiology, 131 (4). pp. 1671-1680. ISSN 00320889 (ISSN)

URL: https://www.ncbi.nlm.nih.gov/pubmed/12692326
DOI: 10.1104/pp.102.017434

Abstract

Expression of KNOX (KNOTTED1-like homeobox) genes in the shoot apical meristem of Arabidopsis is required for maintenance of a functional meristem, whereas exclusion of KNOX gene expression from leaf primordia is required for the elaboration of normal leaf morphology. We have constructed a steroid-inducible system to regulate both the amount and timing of KN1 (KNOTTED1) misexpression in Arabidopsis leaves. We demonstrate that lobed leaf morphology is produced in a dose-dependent manner, indicating that the amount of KN1 quantitatively affects the severity of lobing. The KN1-glucocorticoid receptor fusion protein is not detected in leaves in the absence of steroid induction, suggesting that it is only stable when associated with steroid in an active state. By using a second inducible fusion protein to mark exposure of leaf primordia to the steroid, we determined the stage of leaf development that produces lobed leaves in response to KN1. Primordia as old as plastochron 7 and as young as plastochron 2 were competent to respond to KN1.

Item Type: Paper
Uncontrolled Keywords: Genes Glucose Proteins Apical meristem Plants botany Arabidopsis Dexamethasone Gene Expression Regulation, Plant Homeodomain Proteins Phenotype Plant Leaves Plant Proteins Seedling
Subjects: organism description > plant > Arabidopsis
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > knotted1
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > knotted1
CSHL Authors:
Communities: CSHL labs > Jackson lab
Depositing User: CSHL Librarian
Date: 2003
Date Deposited: 03 Apr 2012 19:30
Last Modified: 11 Jan 2017 21:48
PMCID: PMC166923
Related URLs:
URI: http://repository.cshl.edu/id/eprint/25910

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