A Resource of Cre Driver Lines for Genetic Targeting of GABAergic Neurons in Cerebral Cortex

Taniguchi, H., He, M., Wu, P., Kim, S., Paik, R., Sugino, K., Kvitsani, D., Fu, Y., Lu, J., Lin, Y., Miyoshi, G., Shima, Y., Fishell, G., Nelson, S. B., Huang, Z.  J. (2011) A Resource of Cre Driver Lines for Genetic Targeting of GABAergic Neurons in Cerebral Cortex. Neuron, 71 (6). pp. 995-1013. ISSN 0896-6273

URL: http://www.ncbi.nlm.nih.gov/pubmed/21943598
DOI: 10.1016/j.neuron.2011.07.026

Abstract

Summary A key obstacle to understanding neural circuits in the cerebral cortex is that of unraveling the diversity of GABAergic interneurons. This diversity poses general questions for neural circuit analysis: how are these interneuron cell types generated and assembled into stereotyped local circuits and how do they differentially contribute to circuit operations that underlie cortical functions ranging from perception to cognition? Using genetic engineering in mice, we have generated and characterized approximately 20 Cre and inducible CreER knockin driver lines that reliably target major classes and lineages of GABAergic neurons. More select populations are captured by intersection of Cre and Flp drivers. Genetic targeting allows reliable identification, monitoring, and manipulation of cortical GABAergic neurons, thereby enabling a systematic and comprehensive analysis from cell fate specification, migration, and connectivity, to their functions in network dynamics and behavior. As such, this approach will accelerate the study of GABAergic circuits throughout the mammalian brain.

Item Type: Paper
Uncontrolled Keywords: INTERNEURON DIVERSITY SERIES interneuron diversity series CENTRAL-NERVOUS-SYSTEM central nervous system CORTICAL INTERNEURONS cortical interneurons HIPPOCAMPAL INTERNEURONS hippocampal interneurons IN-VIVO in-vivo SOMATOSENSORY CORTEX somatosensory cortex RAT rat EXPRESSION expression CELLS cells TRANSCRIPTION transcription
Subjects: bioinformatics > genomics and proteomics > small molecules > GABAergic
organs, tissues, organelles, cell types and functions > tissues types and functions > cerebral cortex
neurobiology
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
CSHL Authors:
Communities: CSHL labs > Huang lab
CSHL Post Doctoral Fellows
Depositing User: CSHL Librarian
Date Deposited: 10 Apr 2012 16:38
Last Modified: 07 Apr 2015 16:11
PMCID: PMC3779648
Related URLs:
URI: http://repository.cshl.edu/id/eprint/25814

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving