Cre/loxP recombination-activated neuronal markers in mouse neocortex and hippocampus

Huang, Z. J., Yu, W., Lovett, C., Tonegawa, S. (2002) Cre/loxP recombination-activated neuronal markers in mouse neocortex and hippocampus. Genesis, 32 (3). pp. 209-217. ISSN 1526954X (ISSN)

URL: http://www.ncbi.nlm.nih.gov/pubmed/11892010
DOI: 10.1002/gene.10054

Abstract

A new strategy for visualizing neuronal cell morphology of mouse brain based on Cre/loxP recombination-activated gene expression is described. A "reporter" transgenic line was generated which expressed a fusion gene encoding a dendrite-targeted green fluorescent protein (MAP2-GFP) upon deletion of a transcription/translation STOP (transcription and translation stop signal) cassette. Cre transgenic "deleter" lines were established that activated reporter gene expression at various frequencies in pyramidal neurons in the forebrain. A deleter line was identified which activated a MAP2-GFP reporter gene at very low frequency (less than 0.1% of pyramidal neurons) and allowed the visualization of dendritic structures of individual neocortical and hippocampal pyramidal neurons. In addition, vertical "columns" of pyramidal neurons in the neocortex were labeled in these mice. In a second deleter line, a MAP2-GFP reporter gene was selectively activated in pyramidal neurons of the CA-1 subregion of the hippocampus in young mice. With its combinatorial property, this binary recombination-activated neuronal marker system should facilitate the study of detailed morphology, connectivity, and plasticity of defined classes of live neurons in vitro and in vivo. © 2002 Wiley-Liss, Inc.

Item Type: Paper
Uncontrolled Keywords: Cre/loxP Green fluorescent protein GFP Neocortex Recombination cre recombinase gene product green fluorescent protein unclassified drug animal tissue article cell structure controlled study dendrite forebrain fusion gene gene activation gene cassette gene expression gene frequency gene targeting genetic recombination genetic transcription hippocampus marker gene mouse nerve cell nonhuman plasticity priority journal pyramidal nerve cell reporter gene RNA translation signal transduction Animals Biological Markers Gene Expression Regulation Gene Transfer Techniques Integrases Mice Mice, Transgenic Microscopy, Confocal Neuronal Plasticity Neurons Recombination, Genetic Staining and Labeling Viral Proteins
Subjects: Investigative techniques and equipment
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > green fluorescent protein
organism description > animal > mammal > rodent > mouse
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons

organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > pyramidal neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > pyramidal neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > pyramidal neurons
CSHL Authors:
Communities: CSHL labs > Huang lab
Depositing User: CSHL Librarian
Date Deposited: 10 Apr 2012 15:45
Last Modified: 10 May 2013 19:01
Related URLs:
URI: http://repository.cshl.edu/id/eprint/25807

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