Cell-Type-Based Analysis of MicroRNA Profiles in the Mouse Brain

He, M., Liu, Y., Wang, X., Zhang, M. Q., Hannon, G. J., Huang, Z. J. (2012) Cell-Type-Based Analysis of MicroRNA Profiles in the Mouse Brain. Neuron, 73 (1). pp. 35-48. ISSN 1097-4199 (Electronic)0896-6273 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/22243745
DOI: 10.1016/j.neuron.2011.11.010

Abstract

MicroRNAs (miRNA) are implicated in brain development and function but the underlying mechanisms have been difficult to study in part due to the cellular heterogeneity in neural circuits. To systematically analyze miRNA expression in neurons, we have established a miRNA tagging and affinity-purification (miRAP) method that is targeted to cell types through the Cre-loxP binary system in mice. Our studies of the neocortex and cerebellum reveal the expression of a large fraction of known miRNAs with distinct profiles in glutamatergic and GABAergic neurons and subtypes of GABAergic neurons. We further detected putative novel miRNAs, tissue or cell type-specific strand selection of miRNAs, and miRNA editing. Our method thus will facilitate a systematic analysis of miRNA expression and regulation in specific neuron types in the context of neuronal development, physiology, plasticity, pathology, and disease models, and is generally applicable to other cell types and tissues.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > small molecules > GABAergic
Investigative techniques and equipment
biotechnology > chromatography
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > miRNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > miRNA

organism description > animal > mammal > rodent > mouse
organs, tissues, organelles, cell types and functions > tissues types and functions > visual cortex
CSHL Authors:
Communities: CSHL labs > Hannon lab
CSHL labs > Huang lab
CSHL labs > Zhang lab
CSHL Post Doctoral Fellows
Depositing User: CSHL Librarian
Date Deposited: 10 Apr 2012 18:07
Last Modified: 10 May 2013 14:58
PMCID: PMC3270494
Related URLs:
URI: http://repository.cshl.edu/id/eprint/25797

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