Experience and activity-dependent maturation of perisomatic GABAergic innervation in primary visual cortex during a postnatal critical period

Chattopadhyaya, B., Di Cristo, G., Higashiyama, H., Knott, G. W., Kuhlman, S. J., Welker, E., Huang, Z. J. (2004) Experience and activity-dependent maturation of perisomatic GABAergic innervation in primary visual cortex during a postnatal critical period. Journal of Neuroscience, 24 (43). pp. 9598-611. ISSN 0270-6474

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URL: http://www.ncbi.nlm.nih.gov/pubmed/15509747
DOI: 10.1523/jneurosci.1851-04.2004

Abstract

The neocortical GABAergic network consists of diverse interneuron cell types that display distinct physiological properties and target their innervations to subcellular compartments of principal neurons. Inhibition directed toward the soma and proximal dendrites is crucial in regulating the output of pyramidal neurons, but the development of perisomatic innervation is poorly understood because of the lack of specific synaptic markers. In the primary visual cortex, for example, it is unknown whether, and to what extent, the formation and maturation of perisomatic synapses are intrinsic to cortical circuits or are regulated by sensory experience. Using bacterial artificial chromosome transgenic mice that label a defined class of perisomatic synapses with green fluorescent protein, here we show that perisomatic innervation developed during a protracted postnatal period after eye opening. Maturation of perisomatic innervation was significantly retarded by visual deprivation during the third, but not the fifth, postnatal week, implicating an important role for sensory input. To examine the role of cortical intrinsic mechanisms, we developed a method to visualize perisomatic synapses from single basket interneurons in cortical organotypic cultures. Characteristic perisomatic synapses formed through a stereotyped process, involving the extension of distinct terminal branches and proliferation of perisomatic boutons. Neuronal spiking in organotypic cultures was necessary for the proliferation of boutons and the extension, but not the maintenance, of terminal branches. Together, our results suggest that although the formation of perisomatic synapses is intrinsic to the cortex, visual experience can influence the maturation and pattern of perisomatic innervation during a postnatal critical period by modulating the level of neural activity within cortical circuits.

Item Type: Paper
Uncontrolled Keywords: Action Potentials physiology Animals Chromosomes Artificial, Bacterial DNA Repair Glutamate Decarboxylase genetics Green Fluorescent Proteins genetics Interneurons physiology Isoenzymes genetics Mice Mice, Transgenic Neural Inhibition physiology Neuronal Plasticity physiology Presynaptic Terminals physiology Pyramidal Cells physiology Recombinant Fusion Proteins Sensory Deprivation physiology Synapses physiology Tetrodotoxin pharmacology Time Factors Tissue Culture Techniques Vision physiology Visual Cortex growth & development physiology gamma-Aminobutyric Acid physiology
Subjects: bioinformatics > genomics and proteomics > small molecules > GABAergic
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
organism description > animal > mammal > rodent > mouse
organs, tissues, organelles, cell types and functions > tissues types and functions > neural networks
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > transgenic animal
organs, tissues, organelles, cell types and functions > tissues types and functions > visual cortex
CSHL Authors:
Communities: CSHL labs > Huang lab
Depositing User: CSHL Librarian
Date Deposited: 11 Apr 2012 16:03
Last Modified: 10 May 2013 18:52
Related URLs:
URI: http://repository.cshl.edu/id/eprint/25790

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