Immunocytochemical localization of casein kinase II during interphase and mitosis

Yu, I. J., Spector, D. L., Bae, Y.-S., Marshak, D. R. (1991) Immunocytochemical localization of casein kinase II during interphase and mitosis. J Cell Biol, 114 (6). pp. 1217-32. ISSN 0021-9525

[img]
Preview
PDF (Paper)
Spector J Cell Biol 1991.pdf - Published Version

Download (1936Kb) | Preview
URL: http://www.ncbi.nlm.nih.gov/pubmed/1894695
DOI: 10.1083/jcb.114.6.1217

Abstract

We have developed specific antibodies to synthetic peptide antigens that react with the individual subunits of casein kinase II (CKII). Using these antibodies, we studied the localization of CKII in asynchronous HeLa cells by immunofluorescence and immunoelectron microscopy. Further studies were done on HeLa cells arrested at the G1/S transition by hydroxyurea treatment. Our results indicate that the CKII alpha and beta subunits are localized in the cytoplasm during interphase and are distributed throughout the cell during mitosis. Further electron microscopic investigation revealed that CKII alpha subunit is associated with spindle fibers during metaphase and anaphase. In contrast, the CKII alpha' subunit is localized in the nucleus during G1 and in the cytoplasm during S. Taken together, our results suggest that CKII may play significant roles in cell division control by shifting its localization between the cytoplasm and nucleus.

Item Type: Paper
Additional Information:
Uncontrolled Keywords: Amino Acid Sequence Animals Antibodies diagnostic use Brain enzymology Casein Kinases Cattle Fluorescent Antibody Technique Hela Cells cytology enzymology ultrastructure Humans Hydroxyurea pharmacology Interphase physiology Liver enzymology Microscopy Immunoelectron Mitosis drug effects physiology Molecular Sequence Data Organelles enzymology ultrastructure Peptides chemical synthesis immunology Protein Kinases analysis immunology isolation & purification
Subjects: Investigative techniques and equipment
Investigative techniques and equipment > microscopy > electron microscopy
Investigative techniques and equipment > microscopy > flourescence microscopy
Investigative techniques and equipment > microscopy > immunoflourescence microscopy
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase
Investigative techniques and equipment > microscopy
CSHL Authors:
Communities: CSHL labs > Spector lab
Depositing User: Brian Soldo
Date Deposited: 30 Mar 2012 22:43
Last Modified: 29 Jan 2015 20:45
Related URLs:
URI: http://repository.cshl.edu/id/eprint/25778

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving