Time-resolved analysis and visualization of dynamic processes in living cells

Tvarusko, W., Bentele, M., Misteli, Tom, Rudolf, R., Kaether, C., Spector, David L., Gerdes, H. H., Eils, R. (1999) Time-resolved analysis and visualization of dynamic processes in living cells. Proceedings of the National Academy of Sciences of the United States of America, 96 (14). pp. 7950-5. ISSN 0027-8424

URL: http://www.ncbi.nlm.nih.gov/pubmed/10393928
DOI: 10.1073/pnas.96.14.7950

Abstract

Recent development of in vivo microscopy techniques, including green fluorescent proteins, has allowed the visualization of a wide range of dynamic processes in living cells. For quantitative and visual interpretation of such processes, new concepts for time-resolved image analysis and continuous time-space visualization are required. Here, we describe a versatile and fully automated approach consisting of four techniques, namely highly sensitive object detection, fuzzy logic-based dynamic object tracking, computer graphical visualization, and measurement in time-space. Systematic model simulations were performed to evaluate the reliability of the automated object detection and tracking method. To demonstrate potential applications, the method was applied to the analysis of secretory membrane traffic and the functional dynamics of nuclear compartments enriched in pre-mRNA splicing factors.

Item Type: Paper
Additional Information:
Uncontrolled Keywords: Animals Biology methods Cell Physiology Cercopithecus aethiops Chromogranins analysis genetics Computer Simulation Green Fluorescent Proteins Humans Luminescent Proteins analysis genetics Microscopy methods Models Biological Recombinant Fusion Proteins analysis Time Factors Transfection Vero Cells
Subjects: Investigative techniques and equipment
Investigative techniques and equipment > optical devices
bioinformatics > genomics and proteomics > computers > computer software
Investigative techniques and equipment > optical devices > microscope techniques or equipment
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > splicing factor
CSHL Authors:
Communities: CSHL labs > Spector lab
Depositing User: Brian Soldo
Date Deposited: 30 Mar 2012 22:43
Last Modified: 29 Jan 2015 21:14
Related URLs:
URI: http://repository.cshl.edu/id/eprint/25773

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