The v- and c-myc oncogene proteins colocalize in situ with small nuclear ribonucleoprotein particles

Spector, D. L., Watt, R. A., Sullivan, N. F. (1987) The v- and c-myc oncogene proteins colocalize in situ with small nuclear ribonucleoprotein particles. Oncogene, 1 (1). pp. 5-12. ISSN 0950-9232

URL: http://www.ncbi.nlm.nih.gov/pubmed/2963988

Abstract

The intranuclear distribution of the v-myc and c-myc oncogene proteins were studied by immunofluorescence and immunoelectron microscopy. The nuclear distribution pattern of these proteins is shown to be identical to the distribution of small nuclear ribonucleoprotein particles (snRNPs). Colocalization was observed in cells expressing either the v- or c-myc proteins or in cells microinjected with the recombinant human c-myc protein. Immunolocalization studies revealed the v-myc protein and snRNPs to be concentrated within a nuclear network which excludes the nucleolus, nuclear pore-lamina complex, and portions of the nucleoplasm which contain the bulk of DNA. These results identify a nuclear region enriched in the myc-oncogene protein and snRNPs and raise the possibility that these nuclear constituents may function in related processes.

Item Type: Paper
Additional Information:
Uncontrolled Keywords: Animals Cell Compartmentation Cell Nucleus metabolism ultrastructure Coturnix Fluorescent Antibody Technique Humans Microscopy, Electron Oncogene Proteins, Viral metabolism Proto-Oncogene Proteins metabolism RNA Processing Post Transcriptional Rats Ribonucleoproteins metabolism Ribonucleoproteins Small Nuclear
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > Myc
Investigative techniques and equipment > microscopy > immunoflourescence microscopy
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > oncogene
CSHL Authors:
Communities: CSHL labs > Spector lab
Depositing User: Brian Soldo
Date Deposited: 30 Mar 2012 22:41
Last Modified: 29 Jan 2015 20:49
URI: http://repository.cshl.edu/id/eprint/25761

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