Redistribution of U-snRNPs during mitosis

Spector, D. L., Smith, H. C. (1986) Redistribution of U-snRNPs during mitosis. Experimental Cell Research, 163 (1). pp. 87-94.

URL: http://www.ncbi.nlm.nih.gov/pubmed/2935418
DOI: 10.1016/0014-4827(86)90560-4

Abstract

In this study we examined the distribution of U-snRNPs during mitosis using a monoclonal antibody which recognizes the 28 kD protein associated with U-snRNP particles. During interphase U-snRNPs are concentrated within discrete intranuclear protein clusters. The onset of mitosis is accompained by a breakdown of these nuclear protein clusters and U-snRNPs are redistributed between the condensing chromosomes. During metaphase and anaphase, two populations of U-snRNPs can be identified: those associated with the chromosome surface and a 'free' form distributed throughout the cytoplasm. During telophase, the reformation of nuclear protein clusters appears to be associated with the chromosome surface and coincides with chromatin decondensation. These studies suggest that the interphase assembly of nuclear domains containing U-snRNPs is sensitive to the transcriptionally active or decondensed state of chromatin.

Item Type: Paper
Uncontrolled Keywords: Anaphase Animals Antibodies Monoclonal Cell Line Cell Nucleolus analysis Cell Nucleus analysis Chromosomes analysis Cytoplasm analysis Fluorescent Antibody Technique Interphase Metaphase Mitosis Prophase Ribonucleoproteins analysis immunology Ribonucleoproteins Small Nuclear Telophase
Subjects: organs, tissues, organelles, cell types and functions > organelles, types and functions > mitosis
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > RNP's
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > RNP's > snRNP's
CSHL Authors:
Communities: CSHL labs > Spector lab
Depositing User: Brian Soldo
Date Deposited: 04 Apr 2012 20:22
Last Modified: 29 Jan 2015 20:51
Related URLs:
URI: http://repository.cshl.edu/id/eprint/25758

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