Silver staining, immunofluorescence, and immunoelectron microscopic localization of nucleolar phosphoproteins B23 and C23

Spector, D. L., Ochs, R. L., Busch, H. (1984) Silver staining, immunofluorescence, and immunoelectron microscopic localization of nucleolar phosphoproteins B23 and C23. Chromosoma, 90 (2). pp. 139-48. ISSN 0009-5915 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/6206987
DOI: 10.1007/BF00292451

Abstract

Nucleolar organizer region (NOR)-specific silver staining and immunolocalization of nucleolar phosphoproteins B23 and C23 were compared in Novikoff hepatoma ascites cells. Silver staining and protein C23 immunostaining were both localized in the fibrillar shell surrounding the fibrillar center and in the fibrillar center. During mitosis, silver staining and protein C23 were localized at the NORs. Therefore, protein C23 and the silver-staining protein both seem to be associated with rDNA-containing structures (Mirre and Stahl 1981). A comparison of toluidine blue staining specific for RNA and B23 immunostaining demonstrated that protein B23 was associated with RNA-containing regions of the nucleolus and was absent from the fibrillar centers. Localization of these proteins and their functions are discussed in relation to the organization of the nucleolus.

Item Type: Paper
Additional Information:
Uncontrolled Keywords: Animals Cell Nucleolus ultrastructure Fluorescent Antibody Technique Liver Neoplasms Experimental ultrastructure Microscopy, Electron Nuclear Proteins Rats Ribonucleoproteins analysis Silver Staining and Labeling Tolonium Chloride
Subjects: Investigative techniques and equipment > staining techniques
CSHL Authors:
Communities: CSHL labs > Spector lab
Depositing User: Brian Soldo
Date Deposited: 30 Mar 2012 22:40
Last Modified: 29 Jan 2015 20:58
Related URLs:
URI: http://repository.cshl.edu/id/eprint/25754

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