A novel synaptic transmission mediated by a PACAP-like neuropeptide in Drosophila

Zhong, Y., Pena, L. A. (1995) A novel synaptic transmission mediated by a PACAP-like neuropeptide in Drosophila. Neuron, 14 (3). pp. 527-36. ISSN 0896-6273 (Print)

Abstract

Neuropeptide-mediated transmission was analyzed at Drosophila larval body-wall neuromuscular junctions. Focal application of vertebrate pituitary adenylyl cyclase-activating polypeptide (PACAP38) to the neuromuscular junction region triggered two temporally distinct muscle responses: an immediate depolarization followed by a large enhancement of K+ current. This late enhancement occurred many minutes after the early depolarization. High frequency stimulation of motor nerve fibers evoked a postsynaptic response mimicking that induced by PACAP38. This evoked response was desensitized by preincubation of the preparation with PACAP38. PACAP38-like immunoreactivity was also found in the Drosophila CNS and at almost all larval neuromuscular junctions. Moreover, an immunoreactive band that compares well with PACAP38 in size was identified in Western blot. These results demonstrate that a PACAP-like peptide may function in invertebrates and that a neuropeptide can evoke two distinct postsynaptic responses, each separated by up to 15 min. In addition, this initial electrophysiological study provides a basis for genetic analysis of neuropeptide function in Drosophila.

Item Type: Paper
Uncontrolled Keywords: Animals Blotting Western Drosophila Electric Stimulation Glutamic Acid pharmacology Immunohistochemistry Larva Motor Neurons physiology Nerve Fibers physiology Neuromuscular Junction drug effects physiology Neuropeptides biosynthesis pharmacology physiology Neurotransmitter Agents physiology Patch Clamp Techniques patch-clamp Pituitary Adenylate Cyclase-Activating Polypeptide Potassium Channels drug effects physiology Synaptic Transmission drug effects physiology Vertebrates
Subjects: organism description > animal > insect > Drosophila
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons

Investigative techniques and equipment > patch-clamp recording
Investigative techniques and equipment > recording devices > patch-clamp recording
CSHL Authors:
Communities: CSHL labs > Zhong lab
Depositing User: CSHL Librarian
Date Deposited: 29 Mar 2012 20:06
Last Modified: 29 Mar 2012 20:06
URI: http://repository.cshl.edu/id/eprint/25574

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