Detecting gene copy number fluctuations in tumor cells by microarray analysis of genomic representations

Lucito, R., West, J., Reiner, A., Alexander, J., Esposito, D., Mishra, B., Powers, S., Norton, L., Wigler, M. H. (November 2000) Detecting gene copy number fluctuations in tumor cells by microarray analysis of genomic representations. Genome Research, 10 (11). pp. 1726-1736. ISSN 1088-9051

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URL: https://www.ncbi.nlm.nih.gov/pubmed/11076858
DOI: 10.1101/gr.138300

Abstract

In this work, we explore the use of representations in conjunction with DNA microarray technology to measure gene copy number changes in cancer. We demonstrate that arrays of DNA probes derived From low-complexity representations can be used to detect amplifications, deletions, and polymorphic differences when hybridized to representations of genomic DNA. The method is both reproducible and verifiable, and is applicable even to microscopic amounts of primary tumors. We also present a mathematical model for array performance that is useful for designing and understanding DNA microarray hybridization protocols. The future applications and challenges of this approach are discussed.

Item Type: Paper
Uncontrolled Keywords: CDNA MICROARRAYS CDNA microarrays DNA CANCER Cancer
Subjects: diseases & disorders > cancer
bioinformatics > genomics and proteomics > Validation and Standardization > Microarray Data Standardization and Validation
bioinformatics > genomics and proteomics > analysis and processing > microarray gene expression processing
Investigative techniques and equipment > assays
bioinformatics > computational biology
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > copy number variants
CSHL Authors:
Communities: CSHL labs > Lucito lab
CSHL labs > Powers lab
CSHL labs > Wigler lab
Depositing User: Brian Soldo
Date: November 2000
Date Deposited: 29 Mar 2012 15:18
Last Modified: 04 Jan 2019 20:11
PMCID: PMC310939
Related URLs:
URI: http://repository.cshl.edu/id/eprint/25555

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