High-content analysis of cancer genome DNA alterations

Degenhardt, Y. Y., Wooster, R., McCombie, R. W., Lucito, R., Powers, S. (2008) High-content analysis of cancer genome DNA alterations. Current Opinion in Genetics & Development, 18 (1). pp. 68-72.

URL: http://www.ncbi.nlm.nih.gov/pubmed/18339543
DOI: 10.1016/j.gde.2008.01.005

Abstract

New technologies as well as concerted brute-force approaches have increased the content (number of genes) that can be characterized for genomic DNA alterations. Recent advances include the detection of activating point mutations in key kinase genes (BRAF, EGFR, and PIK3CA) in multiple cancer types: preliminary insight into the entire repertoire of genes that can be mutated in cancer; the discovery of new oncogenes by high-resolution profiling of DNA copy number alterations; and the bioinformatic-driven discovery of oncogenic gene fusions. High-content promoter methylation detection systems have been used to discover additional methylated genes and have provided evidence for a stem cell origin for certain tumors. Some of these advances have had significant impact on the development and clinical testing of new therapeutics.

Item Type: Paper
Subjects: diseases & disorders > cancer
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA methylation
bioinformatics > genomics and proteomics > annotation > gene expression profiling annotation
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > copy number variants
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mutations
CSHL Authors:
Communities: CSHL labs > Lucito lab
CSHL labs > McCombie lab
CSHL labs > Powers lab
Depositing User: Brian Soldo
Date Deposited: 28 Mar 2012 18:52
Last Modified: 11 Apr 2013 19:27
PMCID: PMC2729060
Related URLs:
URI: http://repository.cshl.edu/id/eprint/25541

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