Comparison of the X-ray structure of native rubredoxin from Pyrococcus furiosus with the NMR structure of the zinc-substituted protein

Blake, P. R., Day, M. W., Hsu, B. T., Joshua-Tor, L., Park, J. B., Hare, D. R., Adams, M. W., Rees, D. C., Summers, M. F. (1992) Comparison of the X-ray structure of native rubredoxin from Pyrococcus furiosus with the NMR structure of the zinc-substituted protein. Protein Sci, 1 (11). pp. 1522-5. ISSN 0961-8368 (Print)0961-8368 (Linking)

[img]
Preview
PDF (Paper)
Comparison of the X-ray structure of native.pdf - Published Version

Download (3240Kb) | Preview
URL: https://www.ncbi.nlm.nih.gov/pubmed/1303770
DOI: 10.1002/pro.5560011113

Abstract

The three-dimensional X-ray structures of the oxidized and reduced forms of rubredoxin from Pyrococcus furiosus, determined at -161 degrees C, and the NMR structure of the zinc-substituted protein, determined in solution at 45 degrees C, are compared. The NMR and X-ray structures, which were determined independently, are very similar and lead to similar conclusions regarding the interactions that confer hyperthermostability.

Item Type: Paper
Uncontrolled Keywords: Amino Acid Sequence Archaea metabolism Magnetic Resonance Spectroscopy methods Models Molecular Protein Structure Secondary Rubredoxins chemistry metabolism X-Ray Diffraction methods Zinc metabolism
Subjects: Investigative techniques and equipment > X-Ray Diffraction
Investigative techniques and equipment > x ray crystallography
CSHL Authors:
Communities: CSHL labs > Joshua-Tor lab
Depositing User: CSHL Librarian
Date Deposited: 20 Mar 2012 19:14
Last Modified: 29 Aug 2017 20:20
PMCID: PMC2142123
Related URLs:
URI: http://repository.cshl.edu/id/eprint/25507

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving