NADP regulates the yeast GAL induction system

Kumar, P. R., Yu, Y., Sternglanz, R., Johnston, S. A., Joshua-Tor, L. (2008) NADP regulates the yeast GAL induction system. Science, 319 (5866). pp. 1090-2. ISSN 1095-9203 (Electronic)

URL: https://www.ncbi.nlm.nih.gov/pubmed/18292341
DOI: 10.1126/science.1151903

Abstract

Transcriptional regulation of the galactose-metabolizing genes in Saccharomyces cerevisiae depends on three core proteins: Gal4p, the transcriptional activator that binds to upstream activating DNA sequences (UAS(GAL)); Gal80p, a repressor that binds to the carboxyl terminus of Gal4p and inhibits transcription; and Gal3p, a cytoplasmic transducer that, upon binding galactose and adenosine 5'-triphosphate, relieves Gal80p repression. The current model of induction relies on Gal3p sequestering Gal80p in the cytoplasm. However, the rapid induction of this system implies that there is a missing factor. Our structure of Gal80p in complex with a peptide from the carboxyl-terminal activation domain of Gal4p reveals the existence of a dinucleotide that mediates the interaction between the two. Biochemical and in vivo experiments suggests that nicotinamide adenine dinucleotide phosphate (NADP) plays a key role in the initial induction event.

Item Type: Paper
Additional Information:
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription
organism description > yeast > Saccharomyces
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein structure rendering
Investigative techniques and equipment > x ray crystallography
CSHL Authors:
Communities: CSHL labs > Joshua-Tor lab
Depositing User: CSHL Librarian
Date Deposited: 21 Mar 2012 20:11
Last Modified: 05 Sep 2017 16:45
PMCID: PMC2726985
Related URLs:
Dataset ID:
URI: http://repository.cshl.edu/id/eprint/25469

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving