radish encodes a phospholipase-A2 and defines a neural circuit involved in anesthesia-resistant memory

Chiang, A. S., Blum, A., Barditch, J., Chen, Y. H., Chiu, S. L., Regulski, M., Armstrong, J. D., Tully, T., Dubnau, J. T. (2004) radish encodes a phospholipase-A2 and defines a neural circuit involved in anesthesia-resistant memory. Current Biology, 14 (4). pp. 263-272. ISSN 0960-9822

URL: http://www.ncbi.nlm.nih.gov/pubmed/14972677
DOI: 10.1016/j.cub.2004.02.003

Abstract

Background: In both vertebrate and invertebrate animals, anesthetic agents cause retrograde amnesia for recently experienced events. In contrast, older memories are resistant to the same treatments. In Drosophila, anesthesia-resistant memory (ARM) and long-term memory (LTM) are genetically distinct forms of long-lasting memory that exist in parallel for at least a day after training. ARM is disrupted in radish mutants but is normal in transgenic flies overexpressing a CREB repressor transgene. In contrast, LTM is normal in radish mutants but is disrupted in CREB repressor transgenic flies. To date, nothing is known about the molecular, genetic, or cell biological pathways underlying ARM. Results: Here, we report the molecular identification of radish as a phospholipase-A2, providing the first clue about signaling pathways underlying ARM in any animal. An enhancer-trap allele of radish (Cl 33) reveals expression in a novel anatomical pathway. Transgenic expression of PLA2 under control of C133 restores normal levels of ARM to radish mutants, whereas transient disruption of neural activity in C133 neurons inhibits memory retention. Notably, expression of C133 is not in mushroom bodies, the primary anatomical focus of olfactory memory research in Drosophila. Conclusions: Identification of radish as a phospholipase-A2 and the neural expression pattern of an enhancer-trap allele significantly broaden our understanding of the biochemistry and anatomy underlying olfactory memory in Drosophila.

Item Type: Paper
Uncontrolled Keywords: PROTEIN KINASE-C Protein kinase-C LONG TERM MEMORY Long term memory DROSOPHILA MUSHROOM BODIES Drosophila mushroom bodies INDUCED Induced RETROGRADE AMNESIA Retrograde amnesia PASSIVE AVOIDANCE TASK Passive avoidance task ARACHIDONIC ACID Arachidonic acid OLFACTORY Olfactory MEMORY Memory CONSOLIDATED MEMORY Consolidated memory PRODUCE AMNESIA Produce amnesia LIMAX FLAVUS Limax flavus
Subjects: organism description > animal > insect > Drosophila
organism description > animal behavior > memory
CSHL Authors:
Communities: CSHL labs > Dubnau lab
CSHL labs > Tully lab
Watson School > Publications
Depositing User: Brian Soldo
Date Deposited: 23 Mar 2012 13:32
Last Modified: 26 Sep 2014 18:44
Related URLs:
URI: http://repository.cshl.edu/id/eprint/25416

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