Serotonin-mushroom body circuit modulating the formation of anesthesia-resistant memory in Drosophila

Lee, P. T., Lin, H. W., Chang, Y. H., Fu, T. F., Dubnau, J. T., Hirsh, J., Lee, T., Chiang, A. S. (2011) Serotonin-mushroom body circuit modulating the formation of anesthesia-resistant memory in Drosophila. Proceedings of the National Academy of Sciences of the United States of America, 108 (33). pp. 13794-13799. ISSN 0027-8424

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URL: http://www.ncbi.nlm.nih.gov/pubmed/21808003
DOI: 10.1073/pnas.1019483108

Abstract

Pavlovian olfactory learning in Drosophila produces two genetically distinct forms of intermediate-term memories: anesthesia-sensitive memory, which requires the amnesiac gene, and anesthesia-resistant memory (ARM), which requires the radish gene. Here, we report that ARM is specifically enhanced or inhibited in flies with elevated or reduced serotonin (5HT) levels, respectively. The requirement for 5HT was additive with the memory defect of the amnesiac mutation but was occluded by the radish mutation. This result suggests that 5HT and Radish protein act on the same pathway for ARM formation. Three supporting lines of evidence indicate that ARM formation requires 5HT released from only two dorsal paired medial (DPM) neurons onto the mushroom bodies (MBs), the olfactory learning and memory center in Drosophila: (i) DPM neurons were 5HT-antibody immunopositive; (ii) temporal inhibition of 5HT synthesis or release from DPM neurons, but not from other serotonergic neurons, impaired ARM formation; (iii) knocking down the expression of d5HT1A serotonin receptors in alpha/beta MB neurons, which are innervated by DPM neurons, inhibited ARM formation. Thus, in addition to the Amnesiac peptide required for anesthesia-sensitive memory formation, the two DPM neurons also release 5HT acting on MB neurons for ARM formation.

Item Type: Paper
Uncontrolled Keywords: brain olfaction aversive conditioning neurotransmitter long term memory olfactory memory consolidated memory protein kinase neurons mutant melanogaster expression receptors radish
Subjects: organism description > animal > insect > Drosophila
organism description > animal behavior > memory
organs, tissues, organelles, cell types and functions > tissues types and functions > mushroom body
CSHL Authors:
Communities: CSHL labs > Dubnau lab
Depositing User: Brian Soldo
Date Deposited: 22 Mar 2012 19:05
Last Modified: 02 Jan 2018 21:40
PMCID: PMC3158232
Related URLs:
URI: http://repository.cshl.edu/id/eprint/25408

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