SCL and associated proteins distinguish active from repressive GATA transcription factor complexes

Tripic, T., Deng, W., Cheng, Y., Zhang, Y., Vakoc, C. R., Gregory, G. D., Hardison, R. C., Blobel, G. A. (2009) SCL and associated proteins distinguish active from repressive GATA transcription factor complexes. Blood, 113 (10). pp. 2191-2201. ISSN 00064971 (ISSN)

URL: http://www.ncbi.nlm.nih.gov/pubmed/19011221
DOI: 10.1182/blood-2008-07-169417

Abstract

GATA-1 controls hematopoietic development by activating and repressing gene transcription, yet the in vivo mechanisms that specify these opposite activities are unknown. By examining the composition of GATA-1-associated protein complexes in a conditional erythroid rescue system as well as through the use of tiling arrays we detected the SCL/TAL1, LMO2, Ldbl, E2A complex at all positively acting GATA-1-bound elements examined. Similarly, the SCL complex is present at all activating GATA elements in megakaryocytes and mast cells. In striking contrast, at sites where GATA-1 functions as a repressor, the SCL complex is depleted. A DNA-binding defective form of SCL maintains association with a subset of active GATA elements indicating that GATA-1 is a key determinant for SCL recruitment. Knockdown of LMO2 selectively impairs activation but not repression by GATA-1. ETO-2, an SCL-associated protein with the potential for transcription repression, is also absent from GATA-1-repressed genes but, unlike SCL, fails to accumulate at GATA-1-activated genes. Together, these studies identify the SCL complex as a critical and consistent determinant of positive GATA-1 activity in multiple GATA-1-regulated hematopoietic cell lineages. © 2009 by The American Society of Hematology.

Item Type: Paper
Uncontrolled Keywords: binding protein LIM protein transcription factor E2A transcription factor GATA 1 transcription factor TAL1 animal cell article cell lineage controlled study DNA binding erythroid cell gene activation gene function gene identification gene repression genetic association hematopoietic cell knockout gene mast cell megakaryocyte mouse nonhuman priority journal protein depletion repressor gene transcription initiation transcription regulation Animals Basic Helix Loop Helix Transcription Factors Blood Cells DNA-Binding Proteins GATA1 Transcription Factor Gene Expression Regulation Immunoprecipitation Metalloproteins Mice Oligonucleotide Array Sequence Analysis Proto-Oncogene Proteins Transcription Genetic
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene regulation
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene regulation

bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein receptor
CSHL Authors:
Communities: CSHL labs > Vakoc lab
CSHL Cancer Center Shared Resources > DNA Sequencing Service
Depositing User: CSHL Librarian
Date: 2009
Date Deposited: 19 Mar 2012 18:24
Last Modified: 30 Dec 2014 16:44
PMCID: PMC2652367
Related URLs:
URI: http://repository.cshl.edu/id/eprint/25405

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