Proximity among distant regulatory elements at the β-globin locus requires GATA-1 and FOG-1

Vakoc, C. R., Letting, D. L., Gheldof, N., Sawado, T., Bender, M. A., Groudine, M., Weiss, M. J., Dekker, J., Blobel, G. A. (2005) Proximity among distant regulatory elements at the β-globin locus requires GATA-1 and FOG-1. Molecular Cell, 17 (3). pp. 453-462. ISSN 10972765 (ISSN)

URL: http://www.ncbi.nlm.nih.gov/pubmed/15694345
DOI: 10.1016/j.molcel.2004.12.028

Abstract

Recent evidence suggests that long-range enhancers and gene promoters are in close proximity, which might reflect the formation of chromatin loops. Here, we examined the mechanism for DNA looping at the β-globin locus. By using chromosome conformation capture (3C), we show that the hematopoietic transcription factor GATA-1 and its cofactor FOG-1 are required for the physical interaction between the β-globin locus control region (LCR) and the β-major globin promoter. Kinetic studies reveal that GATA-1-induced loop formation correlates with the onset of β-globin transcription and occurs independently of new protein synthesis. GATA-1 occupies the β-major globin promoter normally in fetal liver erythroblasts from mice lacking the LCR, suggesting that GATA-1 binding to the promoter and LCR are independent events that occur prior to loop formation. Together, these data demonstrate that GATA-1 and FOG-1 are essential anchors for a tissue-specific chromatin loop, providing general insights into long-range enhancer function.

Item Type: Paper
Uncontrolled Keywords: beta globin transcription factor transcription factor fog 1 transcription factor GATA 1 unclassified drug article erythroblast fetus liver gene interaction gene locus genetic transcription promoter region protein function protein synthesis regulator gene Animals Base Sequence Binding Sites Carrier Proteins Cell Line DNA DNA-Binding Proteins Enhancer Elements (Genetics) Erythroid-Specific DNA-Binding Factors GATA1 Transcription Factor Genes, Regulator Globins Humans Locus Control Region Mice Mice, Mutant Strains Nuclear Proteins Nucleic Acid Conformation Promoter Regions (Genetics) Protein Binding RNA Polymerase II Transcription Factors
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > Chromatin dynamics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > transcription factor
CSHL Authors:
Communities: CSHL labs > Vakoc lab
Depositing User: CSHL Librarian
Date: 2005
Date Deposited: 19 Mar 2012 19:07
Last Modified: 13 Mar 2013 20:36
Related URLs:
URI: http://repository.cshl.edu/id/eprint/25402

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