Acetylation of GATA-1 is required for chromatin occupancy

Lamonica, J. M., Vakoc, C. R., Blobel, G. A. (2006) Acetylation of GATA-1 is required for chromatin occupancy. Blood, 108 (12). pp. 3736-3738. ISSN 00064971 (ISSN)

URL: http://www.ncbi.nlm.nih.gov/pubmed/16888089
DOI: 10.1182/blood-2006-07-032847

Abstract

All 3 hematopoietic GATA transcription factors, GATA-1, GATA-2, and GATA-3, are acetylated, although the in vivo role of this modification remains unclear. We examined the functions of an acetylation-defective mutant of GATA-1 in maturing erythroid cells. We found that removal of the acetylation sites in GATA-1 does not impair its nuclear localization, steady-state protein levels, or its ability to bind naked GATA elements in vitro. However, chromatin immunoprecipitation (ChIP) experiments revealed that mutant GATA-1 was dramatically impaired in binding to all examined cellular target sites in vivo, including genes that are normally activated and repressed by GATA-1. Together, these results suggest that acetylation regulates chromatin occupancy of GATA-1. These findings point to a novel function for transcription factor acetylation, perhaps by facilitating protein interactions required for stable association with chromatin templates in vivo. © 2006 by The American Society of Hematology.

Item Type: Paper
Uncontrolled Keywords: transcription factor GATA 1 acetylation animal cell article cell maturation chromatin chromatin immunoprecipitation controlled study DNA binding mouse nonhuman priority journal protein stability steady state Amino Acid Substitution Animals Cell Line GATA1 Transcription Factor GATA2 Transcription Factor GATA3 Transcription Factor Mice Mutation Missense Protein Binding Protein Processing, Post-Translational
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > Chromatin dynamics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > transcription factor
CSHL Authors:
Communities: CSHL labs > Vakoc lab
Depositing User: CSHL Librarian
Date: 2006
Date Deposited: 19 Mar 2012 19:24
Last Modified: 13 Mar 2013 20:26
Related URLs:
URI: http://repository.cshl.edu/id/eprint/25392

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