Temporal profile of replication of human chromosomes

Jeon, Y., Bekiranov, S., Karnani, N., Kapranov, P., Ghosh, S., MacAlpine, D., Lee, C., Deog, S. H., Gingeras, T. R., Dutta, A. (2005) Temporal profile of replication of human chromosomes. Proceedings of the National Academy of Sciences of the United States of America, 102 (18). pp. 6419-6424. ISSN 00278424 (ISSN)

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URL: http://www.ncbi.nlm.nih.gov/pubmed/15845769
DOI: 10.1073/pnas.0405088102

Abstract

Chromosomes in human cancer cells are expected to initiate replication from predictably localized origins, firing reproducibly at discrete times in S phase. Replication products obtained from HeLa cells at different stages of S phase were hybridized to cDNA and genome tiling oligonudeotide microarrays to determine the temporal profile of replication of human chromosomes on a genome-wide scale. About 1,000 genes and chromosomal segments were identified as sites containing efficient origins that fire reproducibly. Early replication was correlated with high gene density. An acute transition of gene density from early to late replicating areas suggests that discrete chromatin states dictate early versus late replication. Surprisingly, at least 60% of the interrogated chromosomal segments replicate equally in all quarters of S phase, suggesting that large stretches of chromosomes are replicated by inefficient, variably located and asynchronous origins and forks, producing a pan-S phase pattern of replication. Thus, at least for aneuploid cancer cells, a typical discrete time of replication in S phase is not seen for large segments of the chromosomes. © 2005 by The National Academy of Sciences of the USA.

Item Type: Paper
Uncontrolled Keywords: Chromatin DNA replication Genome wide Human cancer Microarrays complementary DNA aneuploidy article cancer cell cell cycle S phase controlled study DNA microarray gene expression human human cell human chromosome priority journal Chromosomes Human Cluster Analysis DNA, Complementary Hela Cells Humans In Situ Hybridization, Fluorescence Oligonucleotide Array Sequence Analysis Phylogeny Replication Origin S Phase Time Factors
Subjects: diseases & disorders > cancer
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication
bioinformatics > genomics and proteomics > analysis and processing > microarray gene expression processing
CSHL Authors:
Communities: CSHL labs > Gingeras lab
Depositing User: CSHL Librarian
Date: 2005
Date Deposited: 09 Mar 2012 21:31
Last Modified: 11 Jan 2018 20:08
PMCID: PMC1088349
Related URLs:
URI: http://repository.cshl.edu/id/eprint/25302

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