Mycobacterium species identification and rifampin resistance testing with high-density DNA probe arrays

Troesch, A., Nguyen, H., Miyada, C. G., Desvarenne, S., Gingeras, T. R., Kaplan, P. M., Cros, P., Mabilat, C. (1999) Mycobacterium species identification and rifampin resistance testing with high-density DNA probe arrays. Journal of Clinical Microbiology, 37 (1). pp. 49-55. ISSN 00951137 (ISSN)

URL: http://www.ncbi.nlm.nih.gov/pubmed/9854063

Abstract

Species identification within the genus Mycobacterium and subsequent antibiotic susceptibility testing still rely on time-consuming, culture- based methods. Despite the recent development of DNA probes, which greatly reduce assay time, there is a need for a single platform assay capable of answering the multitude of diagnostic questions associated with this genus. We describe the use of a DNA probe array based on two sequence databases: one for the species identification of mycobacteria (82 unique 16S rRNA sequences corresponding to 54 phenotypical species) and the other for detecting Mycobacterium tuberculosis rifampin resistance (rpoB alleles). Species identification or rifampin resistance was determined by hybridizing fluorescently labeled, amplified genetic material generated from bacterial colonies to the array. Seventy mycobacterial isolates from 27 different species and 15 rifampin-resistant M. tuberculosis strains were tested. A total of 26 of 27 species were correctly identified as well as all of the rpoB mutants. This parallel testing format opens new perspectives in terms of patient management for bacterial diseases by allowing a number of genetic tests to be simultaneously run.

Item Type: Paper
Uncontrolled Keywords: rifampicin antibiotic sensitivity article bacterial gene bacterium identification DNA probe mycobacterium tuberculosis nonhuman nucleotide sequence priority journal tuberculosis Antibiotics, Antitubercular DNA Mutational Analysis DNA Probes DNA, Bacterial Drug Resistance Microbial Humans Microbial Sensitivity Tests Mycobacterium Nucleic Acid Hybridization Rifampin RNA, Ribosomal 16S Species Specificity Actinobacteria (class) Bacteria (microorganisms) Corynebacterineae uncultured actinomycete
Subjects: diseases & disorders
organism description > bacteria
diseases & disorders > cancer > drugs and therapies
CSHL Authors:
Communities: CSHL labs > Gingeras lab
Depositing User: CSHL Librarian
Date: 1999
Date Deposited: 13 Mar 2012 14:26
Last Modified: 28 Mar 2014 19:42
PMCID: PMC84166
Related URLs:
URI: http://repository.cshl.edu/id/eprint/25270

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