High density synthetic oligonucleotide arrays

Lipshutz, R. J., Fodor, S. P. A., Gingeras, T. R., Lockhart, D. J. (1999) High density synthetic oligonucleotide arrays. Nature Genetics, 21 (1 SUPP). pp. 20-24. ISSN 10614036 (ISSN)

URL: http://www.ncbi.nlm.nih.gov/pubmed/9915496
DOI: 10.1038/4447

Abstract

Experimental genomics involves taking advantage of sequence information to investigate and understand the workings of genes, cells and organisms. We have developed an approach in which sequence information is used directly to design high-density, two-dimensional arrays of synthetic oligonucleotides. The GeneChip® probe arrays are made using spatially patterned, light- directed combinatorial chemical synthesis, and contain up to hundreds of thousands of different oligonucleotides on a small glass surface. The arrays have been designed and used for quantitative and highly parallel measurements of gene expression, to discover polymorphic loci and to detect the presence of thousands of alternative alleles. Here, we describe the fabrication of the arrays, their design and some specific applications to high-throughput genetic and cellular analysis.

Item Type: Paper
Uncontrolled Keywords: oligonucleotide DNA probe fluorescence gene expression gene locus gene sequence gene technology laboratory test priority journal quantitative assay review sequence analysis Animals Base Sequence Database Management Systems Genotype Humans Oligonucleotide Array Sequence Analysis Oligonucleotides
Subjects: bioinformatics > genomics and proteomics > analysis and processing > microarray gene expression processing
bioinformatics > genomics and proteomics > annotation > sequence annotation
CSHL Authors:
Communities: CSHL labs > Gingeras lab
Depositing User: CSHL Librarian
Date: 1999
Date Deposited: 13 Mar 2012 16:33
Last Modified: 28 Mar 2014 19:40
Related URLs:
URI: http://repository.cshl.edu/id/eprint/25268

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