Cellular gene expression altered by human cytomegalovirus: Global monitoring with oligonucleotide arrays

Zhu, H., Cong, J. P., Mamtora, G., Gingeras, T. R., Shenk, T. (1998) Cellular gene expression altered by human cytomegalovirus: Global monitoring with oligonucleotide arrays. Proceedings of the National Academy of Sciences of the United States of America, 95 (24). pp. 14470-14475. ISSN 00278424 (ISSN) (Public Dataset)

URL: http://www.ncbi.nlm.nih.gov/pubmed/9826724
DOI: 10.1073/pnas.95.24.14470

Abstract

Mechanistic insights to viral replication and pathogenesis generally have come from the analysis of viral gene products, either by studying their biochemical activities and interactions individually or by creating mutant viruses and analyzing their phenotype. Now it is possible to identify and catalog the host cell genes whose mRNA levels change in response to a pathogen. We have used DNA array technology to monitor the level of ≃6,600 human mRNAs in uninfected as compared with human cytomegalovirus-infected cells. The level of 258 mRNAs changed by a factor of 4 or more before the onset of viral DNA replication. Several of these mRNAs encode gene products that might play key roles in virus-induced pathogenesis, identifying them as intriguing targets for further study.

Item Type: Paper
Uncontrolled Keywords: article cytomegalovirus DNA replication gene expression regulation gene mutation human human cell monitoring nucleotide sequence oligonucleotide probe phenotype priority journal regulator gene target cell transcription regulation unindexed sequence virus cell interaction Annexin A1 Base Sequence Cells, Cultured Cyclooxygenase 2 DNA DNA-Binding Proteins Fibroblasts HLA Antigens Humans Isoenzymes Male Membrane Proteins Microphthalmia-Associated Transcription Factor Oligodeoxyribonucleotides Phospholipases A Promoter Regions (Genetics) Prostaglandin-Endoperoxide Synthases Ribonucleoproteins RNA, Messenger Skin Thrombospondins Transcription Factors Transcription Genetic Animalia DNA viruses Human herpesvirus 5
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication
bioinformatics > genomics and proteomics > analysis and processing > microarray gene expression processing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mRNA dynamics
organism description > virus
CSHL Authors:
Communities: CSHL labs > Gingeras lab
Depositing User: CSHL Librarian
Date: 1998
Date Deposited: 13 Mar 2012 16:30
Last Modified: 28 Mar 2014 19:43
PMCID: PMC24397
Related URLs:
Dataset ID:
URI: http://repository.cshl.edu/id/eprint/25267

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