Reduced HIV-1 infectability of CDd4+ lymphocytes from exposed- uninfected individuals: Association with low expression of CCR5 and high production of β-chemokines

Paxton, W. A., Liu, R., Kang, S., Wu, L., Gingeras, T. R., Landau, N. R., MacKay, C. R., Koup, R. A. (1998) Reduced HIV-1 infectability of CDd4+ lymphocytes from exposed- uninfected individuals: Association with low expression of CCR5 and high production of β-chemokines. Virology, 244 (1). pp. 66-73. ISSN 00426822 (ISSN)

URL: http://www.ncbi.nlm.nih.gov/pubmed/9581779
DOI: 10.1006/viro.1998.9082

Abstract

We examined the human immunodeficiency virus type 1 infectability of CD4+ lymphocytes isolated from CCR5 wild-type individuals, individuals heterozygous for the Δ32 allele of CCR5, and HIV-1-exposed but uninfected (EU) individuals who had CD4+ lymphocytes refractory to M-tropic viral replication. None of the EU individuals were found to be heterozygous for the Δ32 allele. The CD4+ lymphocytes isolated from CCR5/Δ32 and EU individuals were less infectable with an M-tropic viral isolate of HIV-1 than CCR5/CCR5 control individuals but were equally as infectable with a T-tropic viral isolate. The restriction to M-tropic viral isolate replication did not associate with any profound genotypic change in the CCR5 gene. CD4+ lymphocytes from CCR5/Δ32 and CCR5/CCR5 EU individuals were more sensitive to the HIV-inhibitory effects of the recombinant β-chemokines RANTES, MIP- 1α, and MIP-1β than were CD4+ lymphocytes from CCR5/CCR5 control individuals. CD4+ lymphocytes from EU individuals also showed increased sensitivity to recombinant β-chemokines and low surface expression of CCR5. A phenotype of low CCR5 expression and high secretion of β-chemokines is associated with reduced infectability of cells by M-tropic HIV-1. This phenotype may also be associated with protection against sexual transmission of HIV-1.

Item Type: Paper
Additional Information:
Uncontrolled Keywords: article genotype human immunodeficiency virus infection lymphocyte lymphocyte subpopulation nonhuman priority journal virus infectivity virus replication virus transmission CD4-Positive T-Lymphocytes Cell Line Transformed Cell Membrane Chemokines HIV Infections HIV-1 Humans RANTES Receptors CCR5 Recombinant Proteins Human immunodeficiency virus Human immunodeficiency virus 1 RNA viruses
Subjects: diseases & disorders > viral diseases > HIV
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
CSHL Authors:
Communities: CSHL labs > Gingeras lab
Depositing User: CSHL Librarian
Date: 1998
Date Deposited: 13 Mar 2012 16:17
Last Modified: 28 Mar 2014 19:47
Related URLs:
URI: http://repository.cshl.edu/id/eprint/25265

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving