Defining subsets of naive and memory B cells based on the ability of their progeny to somatically mutate in vitro

Decker, D. J., Linton, P. J., Zaharevitz, S., Biery, M., Gingeras, T. R., Klinman, N. R. (1995) Defining subsets of naive and memory B cells based on the ability of their progeny to somatically mutate in vitro. Immunity, 2 (2). pp. 195-203. ISSN 10747613 (ISSN)

URL: http://www.ncbi.nlm.nih.gov/pubmed/7534621
DOI: 10.1016/S1074-7613(95)80092-1

Abstract

The increased affinity of memory antibody responses is due largely to the generation and selection of memory B cells that accumulate somatic mutations after initial antigenic stimulation. Further affinity maturation and mutation also accompany subsequent immunizations. Previous studies have suggested that, like primary antibody-forming cell (AFC) clones, secondary AFC do not accumulate further mutations and, therefore, the origins of progressive affinity maturation remain controversial. Here, we report the generation of somatically mutated memory B cell clones in vitro. Our findings confirm the existence of a naive B cell subset whose progeny, rather than generating AFC, somatically mutate and respond to subsequent antigenic stimulation. Interestingly, upon stimulation, a subset of memory B cells also generates antigen-responsive cells that accumulate further somatic mutations.

Item Type: Paper
Uncontrolled Keywords: animal cell animal experiment antibody producing cell antibody response article b lymphocyte cell clone dna sequence immunization memory cell mouse nonhuman phenotype priority journal somatic mutation Amino Acid Sequence Animal Antibody Formation B-Lymphocyte Subsets Base Sequence Cells Cultured Immunologic Memory Lymphocyte Subsets Mice Molecular Sequence Data Mutation RNA Stem Cells
Subjects: organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > immunity
CSHL Authors:
Communities: CSHL labs > Gingeras lab
Depositing User: CSHL Librarian
Date: 1995
Date Deposited: 13 Mar 2012 15:46
Last Modified: 28 Mar 2014 20:06
Related URLs:
URI: http://repository.cshl.edu/id/eprint/25259

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