Use of self-sustained sequence replication amplification reaction to analyze and detect mutations in zidovudine-resistant human immunodeficiency virus

Gingeras, T. R., Prodanovich, P., Latimer, T., Guatelli, J. C., Richman, D. D., Barringer, K. J. (1991) Use of self-sustained sequence replication amplification reaction to analyze and detect mutations in zidovudine-resistant human immunodeficiency virus. Journal of Infectious Diseases, 164 (6). pp. 1066-1074. ISSN 00221899 (ISSN)

URL: http://www.ncbi.nlm.nih.gov/pubmed/1955709
DOI: 10.1093/infdis/164.6.1066

Abstract

Mutations at amino acid positions 67, 70, 215, and 219 in the human immunodeficiency virus type 1 (HIV-1) pol gene correlate with the emergence of resistance to zidovudine (AZT). These four positions were monitored in viral RNA extracted from infected peripheral blood mononuclear cells (PBMC) and viral stocks obtained after coculture with uninfected lymphocytes. Genotype determinations were made using the self-sustained sequence replication (3SR) and differential bead-based sandwich hybridization (BBSH) assay. The hybridization results obtained by 3SR and BBSH analyses were verified by dideoxynucleotide sequencing of the 3SR products. Correlation of 3SR and BBSH with polymerase chain reaction and Southern hybridization analyses of the PBMC and corresponding viral isolates indicated that PBMC and corresponding HIV-1 isolates may differ in their genotypes at the monitored amino acid positions, variations from the wild-type nucleotide sequence may occur proximal to the codons being monitored, and viral isolates possessing the same genotypes at the four monitored amino acid positions showed a threefold variation in their ID50 measurements.

Item Type: Paper
Uncontrolled Keywords: zidovudine amino acid sequence article drug resistance genotype human human immunodeficiency virus 1 human immunodeficiency virus infection mutation nonhuman priority journal virus mutant Base Sequence Blotting Southern DNA Replication Drug Resistance Microbial Genetic Vectors HIV Infections HIV-1 Molecular Sequence Data Nucleic Acid Hybridization Oligonucleotide Probes Plasmids Polymerase Chain Reaction Predictive Value of Tests RNA, Viral Transcription Genetic
Subjects: diseases & disorders > viral diseases > HIV
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNA replication
therapies
CSHL Authors:
Communities: CSHL labs > Gingeras lab
Depositing User: CSHL Librarian
Date: 1991
Date Deposited: 14 Mar 2012 13:31
Last Modified: 28 Mar 2014 20:47
Related URLs:
URI: http://repository.cshl.edu/id/eprint/25252

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