Unique features of the self-sustained sequence replication (3SR) reaction in the in vitro amplification of nucleic acids

Gingeras, T. R., Whitfield, K. M., Kwoh, D. Y. (1990) Unique features of the self-sustained sequence replication (3SR) reaction in the in vitro amplification of nucleic acids. Annales de Biologie Clinique, 48 (7). pp. 498-501. ISSN 00033898 (ISSN)

URL: http://www.ncbi.nlm.nih.gov/pubmed/2278414

Abstract

The development of a transcription-based amplification system and its application to a retrospective analysis of HIV-1-infected clinical samples demonstrated the specificity and sensitivity of this in vitro amplification procedure. The TAS protocol has been modified to mimic the retroviral strategy of replication, resulting in a self-sustained sequence replication (3SR) amplification reaction which operates under isothermal conditions (37°C). The ability to specifically amplify only RNA sequences in the presence of DNA genomic copies containing the same sequence and the rapid kinetics of the 3SR reaction distinguish it from the well-used PCR protocol.

Item Type: Paper
Uncontrolled Keywords: dna replication dna sequence genetic engineering human immunodeficiency virus 1 nonhuman Comparative Study DNA Viral Gene Amplification HIV-1 Nucleic Acid Hybridization Polymerase Chain Reaction Human immunodeficiency virus
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene amplification
CSHL Authors:
Communities: CSHL labs > Gingeras lab
Depositing User: CSHL Librarian
Date: 1990
Date Deposited: 13 Mar 2012 16:47
Last Modified: 31 Mar 2014 13:31
URI: http://repository.cshl.edu/id/eprint/25248

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving