Hybridization properties of immobilized nucleic acids

Gingeras, T. R., Kwoh, D. Y., Davis, G. R. (1987) Hybridization properties of immobilized nucleic acids. Nucleic Acids Research, 15 (13). pp. 5373-5390. ISSN 03051048 (ISSN)

URL: http://www.ncbi.nlm.nih.gov/pubmed/3601675
DOI: 10.1093/nar/15.13.5373

Abstract

The 5′-end attachment of oligonucleotides to dextran supports facilitates the study of the hybridization properties of an immobilized oligonucleotide system. The hybridization properties which were studied include: hybridization capacity and kinetics, hybridization-complex stability, and reagents influencing hybridization efficiency. Results of these experiments reveal that the hybridization efficiencies of support-bound oligonucleotides were 75-80% and 40-50% for single-stranded oligonucleotide targets and long double-stranded targets, respectively. These hybridization efficiencies are dependent upon prehybridizing the support-bound oligonucleotides with dextran sulfate. In addition, comparisons of the relative hybridization efficiencies of the support-bound oligonucleotide and nitrocellulose-based systems have been made which indicate a retention of 13-28% of target sequences on the filters and a detection efficiency of 8-20%. © 1987 IRL Press Limited.

Item Type: Paper
Uncontrolled Keywords: acrylic acid resin DNA oligodeoxyribonucleotide sephacryl s 500 article kinetics nucleic acid hybridization plasmid Acrylic Resins Oligodeoxyribonucleotides Plasmids
Subjects: Investigative techniques and equipment > comparative genomic hybridization
Investigative techniques and equipment > assays > comparative genomic hybridization

Investigative techniques and equipment
CSHL Authors:
Communities: CSHL labs > Gingeras lab
Depositing User: CSHL Librarian
Date: 1987
Date Deposited: 14 Mar 2012 14:09
Last Modified: 31 Mar 2014 13:49
PMCID: PMC305967
Related URLs:
URI: http://repository.cshl.edu/id/eprint/25239

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