Cyclic AMP response element-binding (CREB)-like proteins in a molluscan brain: cellular localization and learning-induced phosphorylation

Ribeiro, M. J., Serfozo, Z., Papp, A., Kemenes, I., O'Shea, M., Yin, J. C. P., Benjamin, P. R., Kemenes, G. (September 2003) Cyclic AMP response element-binding (CREB)-like proteins in a molluscan brain: cellular localization and learning-induced phosphorylation. European Journal of Neuroscience, 18 (5). pp. 1223-1234. ISSN 0953-816X

URL: http://onlinelibrary.wiley.com/doi/10.1046/j.1460-...

Abstract

The phosphorylation and the binding to DNA of the nuclear transcription factor, cyclic adenosine 3',5'-monophosphate (cAMP) response element-binding protein (CREB) are conserved key steps in the molecular cascade leading to the formation of long-term memory (LTM). Here, we characterize, for the first time, a CREB1-like protein in the central nervous system (CNS) of Lymnaea, a model system used widely for the study of the fundamental mechanisms of learning and memory We demonstrate cAMP response element (CRE)-binding activity in CNS protein extracts and show that one of the CRE-binding proteins is recognized by a polyclonal antibody raised to mammalian (human) CREB1. The same antibody detects specific CREB1 immunoreactivity in CNS extracts and in the nuclei of most neurons in the brain. Moreover, phospho-CREB1 -specific immunoreactivity is increased significantly in protein extracts of the CNS by forskolin, an activator of adenylate cyclase. The forskolin-induced increase in phospho-CREB1 immunoreactivity is localized to the nuclei of CNS neurons, some of which have an important role in the formation of LTM. Significantly, classical food-reward conditioning increases phospho-CREB1 immunoreactivity in Lymnaea CNS protein extracts. This increase in immunoreactivity is specific to the ganglia that contain the feeding circuitry, which undergoes cellular changes after classical conditioning. This work establishes the expression of a highly conserved functional CREB1-like protein in the CNS of Lymnaea and opens the way for a detailed analysis of the role of CREB proteins in LTM formation in this model system.

Item Type: Paper
Uncontrolled Keywords: classical conditioning CREB feeding network Lymnaea brain transcription factors LONG-TERM-MEMORY Long-term memory CONDITIONED TASTE-AVERSION conditioned taste aversion CENTRAL PATTERN central pattern GENERATOR generator SNAIL LYMNAEA-STAGNALIS snail Lymnaea stagnalis POND SNAIL pond snail NERVOUS-SYSTEM nervous system SOMATOSTATIN GENE Somatostatin gene FEEDING NETWORK feeding network FACTOR CREB factor CREB TRANSCRIPTION transcription
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein expression > phosphorylation
CSHL Authors:
Depositing User: Leigh Johnson
Date: September 2003
Date Deposited: 07 Mar 2012 19:36
Last Modified: 07 Mar 2012 19:36
URI: http://repository.cshl.edu/id/eprint/25211

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving