Transient Replication of Bpv-1 Requires 2 Viral Polypeptides Encoded by the E1 and E2 Open Reading Frames

Ustav, M., Stenlund, A. (February 1991) Transient Replication of Bpv-1 Requires 2 Viral Polypeptides Encoded by the E1 and E2 Open Reading Frames. Embo Journal, 10 (2). pp. 449-457. ISSN 0261-4189

URL: https://www.ncbi.nlm.nih.gov/pubmed/1846806

Abstract

Bovine papillomavirus (BPV) DNA is maintained as an episome with a constant copy number in transformed cells and is stably inherited. To study BPV replication we have developed a transient replication assay based on a highly efficient electroporation procedure. Using this assay we have determined that in the context of the viral genome two of the viral open reading frames, E1 and E2, are required for replication. Furthermore we show that when produced from expression vectors in the absence of other viral gene products, the full length E2 transactivator polypeptide and a 72 kd polypeptide encoded by the E1 open reading frame in its entirety, are both necessary and sufficient for replication BPV in C127 cells.

Item Type: Paper
Uncontrolled Keywords: DNA REPLICATION replication PAPILLOMAVIRUS Papillomavirus TRANSACTIVATOR transactivator BOVINE PAPILLOMAVIRUS TYPE 1 bovine papillomavirus type 1 EPSTEIN BARR VIRUS Epstein Barr Virus CARBOXY TERMINAL Carboxy terminal DOMAIN domain DNA REPLICATION DNA replication TRANSCRIPTIONAL REGULATION transcriptional regulation TRANSFORMED CELLS transformed cells GENETIC ANALYSIS genetic analysis PROTEINS proteins PLASMIDS plasmids PRODUCT
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication
Investigative techniques and equipment
Investigative techniques and equipment > assays
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein
organism description > virus > papillomavirus
CSHL Authors:
Communities: CSHL labs > Stenlund lab
Depositing User: Brian Soldo
Date: February 1991
Date Deposited: 15 Mar 2012 14:42
Last Modified: 14 Feb 2017 20:42
PMCID: PMC452666
URI: http://repository.cshl.edu/id/eprint/25204

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