Identification of the origin of replication of bovine papillomavirus and characterization of the viral origin recognition factor E1

Ustav, M., Ustav, E., Szymanski, P., Stenlund, A. (December 1991) Identification of the origin of replication of bovine papillomavirus and characterization of the viral origin recognition factor E1. Embo J, 10 (13). pp. 4321-9.

URL: https://www.ncbi.nlm.nih.gov/pubmed/1661672

Abstract

Expression of the viral polypeptides E1 and E2 is necessary and sufficient for replication of BPV in mouse C127 cells. By providing these factors from heterologous expression vectors we have identified a minimal origin fragment from BPV that contains all the sequences required in cis for replication of BPV in short term replication assays. This same sequence is also required for stable replication in the context of the entire viral genome. The identified region is highly conserved between different papillomaviruses, and is unrelated to the previously identified plasmid maintenance sequences. The minimal ori sequence contains a binding site for the viral polypeptide E1, which we identify as a sequence specific DNA binding protein, but surprisingly, an intact binding site for the viral transactivator E2 at the ori is not required. The isolated origin shows an extended host region for replication and replicates efficiently in both rodent and primate cell lines.

Item Type: Paper
Uncontrolled Keywords: Animals Base Sequence Binding Sites Blotting Western Cell Line DNA Fingerprinting DNA Replication DNA Viral biosynthesis metabolism DNA Binding Proteins metabolism Enhancer Elements Genetics Escherichia coli metabolism Gene Expression Genes Viral Mice Molecular Sequence Data Papillomavirus Bovine genetics Plasmids Restriction Mapping Sequence Homology Nucleic Acid Viral Proteins metabolism
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein
organism description > virus > papillomavirus
CSHL Authors:
Communities: CSHL labs > Stenlund lab
Depositing User: Brian Soldo
Date: December 1991
Date Deposited: 15 Mar 2012 14:51
Last Modified: 14 Feb 2017 20:39
PMCID: PMC453185
URI: http://repository.cshl.edu/id/eprint/25202

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