Ustav, E., Ustav, M., Szymanski, P., Stenlund, A. (February 1993) The bovine papillomavirus origin of replication requires a binding site for the E2 transcriptional activator. Proc Natl Acad Sci U S A, 90 (3). pp. 898-902. ISSN 0027-8424 (Print)
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Abstract
The bovine papillomavirus type I transcriptional activator E2 is essential for replication of bovine papillomavirus DNA, yet most of the high-affinity binding sites for E2 are dispensable. Here we demonstrate an absolute requirement for a binding site for the E2 polypeptide as a cis-acting replication element, establishing that site-specific binding of E2 to the origin is a prerequisite for bovine papillomavirus replication in vivo. The position and distance of the E2 binding site relative to the other origin of replication components are flexible, but function at a distance requires high-affinity E2 binding sites. Thus, low-affinity binding sites function only when located close to the origin of replication, while activity at greater distances requires multimerized high-affinity E2 binding sites. The requirement for E2, although different in some respects, shows distinct similarities to what has been termed replication enhancers and may provide insight into the function of this class of DNA replication element.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Base Sequence DNA Mutational Analysis DNA Replication DNA Binding Proteins metabolism Enhancer Elements Genetics genetics Molecular Sequence Data Nucleic Acid Conformation Papillomavirus Bovine genetics growth & development Structure Activity Relationship Transcription Factors genetics Transcription Genetic Viral Proteins metabolism Virus Replication |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein organism description > virus > papillomavirus |
| CSHL Authors: | |
| Communities: | CSHL labs > Stenlund lab |
| Depositing User: | Brian Soldo |
| Date: | 1 February 1993 |
| Date Deposited: | 15 Mar 2012 15:12 |
| Last Modified: | 09 Nov 2017 20:02 |
| PMCID: | PMC45777 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/25200 |
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