Functional interactions between papillomavirus E1 and E2 proteins

Berg, M., Stenlund, A. (May 1997) Functional interactions between papillomavirus E1 and E2 proteins. J Virol, 71 (5). pp. 3853-63. ISSN 0022-538X (Print)

URL: https://www.ncbi.nlm.nih.gov/pubmed/9094661

Abstract

DNA replication of papillomaviruses requires the viral E1 and E2 proteins. These proteins bind cooperatively to the viral origin of replication (ori), which contains binding sites for both proteins, forming an E1-E2-ori complex which is essential for initiation of DNA replication. To map the domains in E2 that are involved in the interaction with E1, we have used chimeric bovine papillomavirus (BPV)/human papillomavirus type 11 (HPV-11) E2 proteins. The results from this study show that both the DNA binding domain and the transactivation domain from BPV E2 independently can interact with BPV E1. However, the roles of these two interactions are different: the interaction between E1 and the activation domain of E2 is necessary and sufficient for cooperativity in binding and for DNA replication; the interaction between E1 and the DNA binding domain of E2 is required only when the binding sites for E1 and E2 are adjacent to each other, and the function of this interaction appears to be to facilitate the interaction between E1 and the transactivation domain of E2. These results indicate that the cooperative binding of E1 and E2 to the BPV ori takes place via a novel two-stage mechanism where one interaction serves as a trigger for the formation of the second, productive, interaction between the two proteins.

Item Type: Paper
Uncontrolled Keywords: Binding Sites DNA metabolism DNA Replication DNA Binding Proteins physiology Viral Proteins physiology Virus Replication
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein
organism description > virus > papillomavirus
CSHL Authors:
Communities: CSHL labs > Stenlund lab
Depositing User: Brian Soldo
Date: May 1997
Date Deposited: 15 Mar 2012 15:28
Last Modified: 14 Feb 2017 20:26
PMCID: PMC191536
Related URLs:
URI: http://repository.cshl.edu/id/eprint/25195

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