Characterization of the DNA-binding domain of the bovine papillomavirus replication initiator E1

Chen, G., Stenlund, A. (April 1998) Characterization of the DNA-binding domain of the bovine papillomavirus replication initiator E1. J Virol, 72 (4). pp. 2567-76. ISSN 0022-538X (Print)

URL: https://www.ncbi.nlm.nih.gov/pubmed/9525573

Abstract

The bovine papillomavirus replication initiator protein E1 is an origin of replication (ori)-binding protein absolutely required for viral DNA replication. In the presence of the viral transcription factor E2, E1 binds to the ori and initiates DNA replication. To understand how the E1 initiator recognizes the ori and how E2 assists in this process, we have expressed and purified a 166-amino-acid fragment which corresponds to the minimal E1 DNA-binding domain (DBD). DNA binding studies using this protein demonstrate that the E1 DBD can bind to the palindromic E1 binding site in several forms but that binding of two monomers, each recognizing one half-site of the E1 palindrome, is the predominant form. This is reminiscent of the binding of the T-antigen DBD to the SV40 ori, and interestingly, the arrangement of E1 binding sites shows striking similarities to the arrangement of T-antigen binding sites in the SV40 ori even though the recognition sequences are unrelated. The E1 DBD is capable of interacting cooperatively with E2; however, the E2 DBD and not the E2 activation domain mediates this interaction. Furthermore, the E2 DBD stimulates binding of two monomers of the E1 DBD to the ori by binding cooperatively with one E1 monomer. Finally, we show that our results concerning the DNA-binding properties of the E1 DBD can be extended to full-length E1.

Item Type: Paper
Uncontrolled Keywords: Animals Binding Sites Cattle DNA metabolism DNA Replication DNA Binding Proteins genetics metabolism Mutagenesis Papillomavirus Bovine metabolism physiology Recombinant Fusion Proteins genetics metabolism Replication Origin Structure-Activity Relationship Viral Proteins genetics metabolism Virus Replication
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein
organism description > virus > papillomavirus
CSHL Authors:
Communities: CSHL labs > Stenlund lab
Depositing User: Brian Soldo
Date: April 1998
Date Deposited: 15 Mar 2012 15:36
Last Modified: 14 Feb 2017 20:25
PMCID: PMC109687
Related URLs:
URI: http://repository.cshl.edu/id/eprint/25193

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