Recruitment and loading of the E1 initiator protein: an ATP-dependent process catalysed by a transcription factor

Sanders, C. M., Stenlund, A. (December 1998) Recruitment and loading of the E1 initiator protein: an ATP-dependent process catalysed by a transcription factor. Embo J, 17 (23). pp. 7044-55. ISSN 0261-4189 (Print)

URL: https://www.ncbi.nlm.nih.gov/pubmed/9843509
DOI: 10.1093/emboj/17.23.7044

Abstract

Initiation of DNA replication critically depends on ori recognition as well as on catalytic activities of the initiator complex. For replication of papillomaviruses the catalytic activities for initiation are provided by the E1 protein. Here, we show that the transcription factor E2 acts to assemble E1 into a complex active for ori distortion in two steps. First, cooperative DNA binding of E1 and E2 generates a sequence-specific ori recognition complex. In the second ATP-dependent step, E2 is displaced and additional E1 molecules are incorporated. The net result is a final complex with low sequence specificity deposited onto a specific sequence in the DNA. This may be a general strategy to accomplish specific positioning of protein complexes with low sequence specificity.

Item Type: Paper
Uncontrolled Keywords: Adenosine Triphosphate metabolism Animals Catalysis Cattle DNA Footprinting DNA-Binding Proteins metabolism Deoxyribonuclease I Magnesium Replication Origin Research Support Non US Govt Research Support US Govt PHS Transcription Factors metabolism Transcription Genetic Viral Proteins metabolism
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > transcription factor
organism description > virus
CSHL Authors:
Communities: CSHL labs > Stenlund lab
Depositing User: Brian Soldo
Date: 1 December 1998
Date Deposited: 15 Mar 2012 15:50
Last Modified: 14 Feb 2017 20:22
PMCID: PMC1171052
Related URLs:
URI: http://repository.cshl.edu/id/eprint/25192

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