The E1 initiator recognizes multiple overlapping sites in the papillomavirus origin of DNA replication

Chen, G., Stenlund, A. (January 2001) The E1 initiator recognizes multiple overlapping sites in the papillomavirus origin of DNA replication. Journal of Virology, 75 (1). pp. 292-302. ISSN 0022-538X

URL: https://www.ncbi.nlm.nih.gov/pubmed/11119599
DOI: 10.1128/jvi.75.1.292-302.2001

Abstract

A common feature of replicator sequences from a variety of organisms is multiple binding sites for an initiator protein. By binding to the replicator, initiators mark the site and contribute to melting or distortion of the DNA. We have defined the recognition sequence for the papillomavirus El initiator and determined the arrangement of binding sites in the viral origin of replication. We show that El recognizes a hexanucleotide sequence which is present in overlapping arrays in virtually all papillomavirus replicators. Binding of the initiator to these sites would result in the formation of a closely packed array of El molecules that wrap around the double helix.

Item Type: Paper
Uncontrolled Keywords: VIRUS-40 CORE ORIGIN virus-40 core origin LARGE TUMOR ANTIGEN E2 TRANSCRIPTIONAL ACTIVATOR BOVINE PAPILLOMAVIRUS T-ANTIGEN BINDING DOMAIN binding domain viral ORIGIN origin TYPE-1 E1 Type-1 HELICASE ACTIVITIES Helicase Activities STRUCTURAL CHANGES
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein
organism description > virus > papillomavirus
CSHL Authors:
Communities: CSHL labs > Stenlund lab
Depositing User: Brian Soldo
Date: January 2001
Date Deposited: 14 Mar 2012 18:09
Last Modified: 14 Feb 2017 20:14
PMCID: PMC113923
Related URLs:
URI: http://repository.cshl.edu/id/eprint/25186

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