Formation of a preinitiation complex by S-phase cyclin CDK-dependent loading of Cdc45p onto chromatin

Zou, L., Stillman, B. (1998) Formation of a preinitiation complex by S-phase cyclin CDK-dependent loading of Cdc45p onto chromatin. Science, 280 (5363). pp. 593-6. ISSN 0036-8075 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/9554851
DOI: 10.1126/science.280.5363.593

Abstract

Cdc45p, a protein essential for initiation of DNA replication, associates with chromatin after "start" in late G1 and during the S phase of the cell cycle. Binding of Cdc45p to chromatin depends on Clb-Cdc28 kinase activity as well as functional Cdc6p and Mcm2p, which suggests that Cdc45p associates with the prereplication complex after activation of S-phase cyclin-dependent kinases (CDKs). As indicated by the timing and the CDK dependence, binding of Cdc45p to chromatin is crucial for commitment to initiation of DNA replication. During S phase, Cdc45p physically interacts with minichromosome maintenance (MCM) proteins on chromatin; however, dissociation of Cdc45p from chromatin is slower than that of MCMs, which indicates that the proteins are released by different mechanisms.

Item Type: Paper
Uncontrolled Keywords: CDC28 Protein Kinase S cerevisiae metabolism Carrier Proteins metabolism Cell Cycle Proteins metabolism Chromatin metabolism Cyclin-Dependent Kinases metabolism Cyclins metabolism DNA Replication DNA, Fungal biosynthesis DNA-Binding Proteins metabolism Enzyme Activation Fungal Proteins metabolism G1 Phase Nuclear Proteins metabolism Origin Recognition Complex S Phase Saccharomyces cerevisiae cytology metabolism Saccharomyces cerevisiae Proteins
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > Chromatin dynamics
CSHL Authors:
Communities: CSHL labs > Stillman lab
Highlight: Stillman, Bruce W.
Depositing User: CSHL Librarian
Date Deposited: 17 Feb 2012 15:20
Last Modified: 20 Jun 2017 19:28
Related URLs:
URI: http://repository.cshl.edu/id/eprint/25151

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